Our Anti-PTPH1 (Ser459) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse PTPH1 (Ser459) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of mouse testicular lysate showing specific immunolabeling of the ~104 kDa PTPH1 phosphorylated at Ser459 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase , 400 units/100ul lysate for 30 minutes, RT).
Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser-459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser-459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
PTPH1
104 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 of human PTPH1, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse
Bovine, Canine, Non-Human Primate, Sheep
AB_2492219
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~104 kDa PTPH1 protein phosphorylated at Ser459. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser459
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
cytoskeletal-associated protein tyrosine phosphatase antibody, protein tyrosine phosphatase H1 antibody, protein tyrosine phosphatase non-receptor type 3 antibody, Protein-tyrosine phosphatase H1 antibody, PTN3_HUMAN antibody, PTP-H1 antibody, PTPH1 antibody, PTPN3 antibody, Tyrosine-protein phosphatase non-receptor type 3 antibody
Hou, S., et al. 2012. p38γ Mitogen-activated protein kinase signals through phosphorylating its phosphatase PTPH1 in regulating ras protein oncogenesis and stress response. Journal of Biological Chemistry, 287(33), pp.27895-27905.
