Anti-Synapsin I (C-terminal region) Antibody (M365)

Name: Anti-Synapsin I (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SM3651
Price: 370.0 USD
Availability: InStock

1 Citation

Our Synapsin I (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Synapsin I (C-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Immunocytochemical labeling of Synapsin I in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Synapsin I (C-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.

Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-Synapsin I (C-terminal region) antibody at 1:250 (lane 1) or 1:1000 (lane 2).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: SM3651

Volume: 100 µL

Variant

Price:

$370.00

Quantity:

Product Details

Target

Synapsin I (C-terminal region)

Target Description

Synapsins are important peripheral membrane proteins involved in synaptic vesicle release during neurotransmission between neurons. Several synapsin variants are generated by alternative splicing of the mRNA from three different genes: synapsin I, II, and III. Synapsins bind to synaptic vesicles via their conserved N-terminal domains. Regulation of synapsins may occur through PKA and CAM kinase I phosphorylation. This regulation occurs concomitantly with membrane excitation and neurotransmitter release into the synaptic cleft. Synapsins bundle actin filaments and interact with multiple calcium binding proteins. Thus, synapsins may crosslink vesicles or other proteins to actin filaments at the presynaptic terminal. In addition, synapsins may interact with each other, since synapsin III has been shown to form heteromultimers with both synapsin I and synapsin II. All synapsins contain a central ATP binding domain and variable C-terminal domains that facilitate distinct regulatory functions.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M365

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

SYN1

Molecular Weight

Antigen

Clone M365 was generated from a sequence corresponding to amino acids in the C-terminal region of mouse synapsin I. This sequence has high homology to human and rat synpasin I.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects an 80 kDa* protein corresponding to the molecular mass of Synapsin I on SDS-PAGE immunoblots of adult mouse brain.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SYN1, SYN1a, SYN1b, SynapsinI, SYNI

UniProt Details

UniProt (Human): P17600
UniProt (Immunogen Species): O88935

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Western Blot: Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
25037702	1:500	Chanaday, N.L., et al. 2015. Glutamate release machinery is altered in the frontal cortex of rats with experimental autoimmune encephalomyelitis. Molecular Neurobiology, 1353-1367.

Western Blot: Rat
PMID	Dilution	Publication
25037702	1:1000	Chanaday, N.L., et al. 2015. Glutamate release machinery is altered in the frontal cortex of rats with experimental autoimmune encephalomyelitis. Molecular Neurobiology, 1353-1367.

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Anti-Synapsin I (C-terminal region) Antibody (M365)

SKU: SM3651

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Synapsin I (C-terminal region) Antibody

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