Anti-CaM Kinase II (Thr286) Antibody

Name: Anti-CaM Kinase II (Thr286) Antibody
Brand: PhosphoSolutions
SKU: p1005-286
Price: 430.0 USD
Availability: InStock

34 Citations

Our Anti-CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, rabbit, and rat CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Astrocytes (1 × 106 cells/ml) were cultured for 24 h without treatment or with METH (10 μM), clade B gp120 (50 ng/ ml), or clade C gp120 (50 ng/ ml) alone or METH in combination with clade B or C gp120. After incubation, the cells were fixed, permeabilized, and stained for (A) DRD-2, (B) phospho-CaMK II (cat. p1005-286) (C) phospho-CaMK IV (green) or GFPA (red) and DAPI (blue). The cells were imaged using a confocal microscope at 630x and analyzed using immunocytochemistry. Image from publication CC-BY-4.0.

Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring CaMKIIα WT and F89G phosphorylation of T286 (cat. p1005-286, 1:2500). Image from publication CC-BY-4.0. PMID: 37648853

Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41–10 μM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853

Depicts increased CaMKII autophosphorylation at Threonine 287 (cat. p1005-286, 1:1000) (normalized to CaMKIIδ expression) following ATX-II exposure in WT and KI cells. Image from publication CC-BY-4.0. PMID: 39077112

Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Species Reactivity

Human, Mouse, Rabbit, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Go to Full Product Details

Data Sheet

Trial Size

Pooled Serum

SKU: p1005-286

Volume: 100 µL

Variant

Price:

$430.00

Quantity:

Product Details

Target

CaM Kinase II (Thr286)

Target Description

Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr-286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr-305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

CAMK2A/B

Molecular Weight

50/60 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rabbit, Rat

Expected Reactivity

Zebrafish

Antibody Registry ID

AB_2492051

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~50 kDa α-CaM Kinase II and the ~60 kDa β-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr286

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody

UniProt Details

UniProt (Human): Q9UQM7
UniProt (Immunogen Species): P11275

NCBI Gene ID

25400

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Rabbit
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
26057350	not listed	Samikkannu, T., et al. 2015. HIV subtypes B and C gp120 and methamphetamine interaction: dopaminergic system implicates differential neuronal toxicity. Scientific reports, 5, p.11130.

Immunocytochemistry: Rat
PMID	Dilution	Publication
32492405	not listed	Li, B., et al. 2020. Neuronal Inactivity Co-opts LTP Machinery to Drive Potassium Channel Splicing and Homeostatic Spike Widening. Cell, S0092-8674(20), p.p. 30575-4.

Immunohistochemistry: Rabbit
PMID	Dilution	Publication
22593045	1:1000	Kothmann, W.W., et al. 2012. Nonsynaptic NMDA Receptors Mediate Activity-Dependent Plasticity of Gap Junctional Coupling in the All Amacrine Cell Network. J. Neurosci. 32(3): 6747-6759.

Western Blot: Human
PMID	Dilution	Publication
37907140	1:1000	Castellanos, LCS, et al. 2023. Redistribution of ASIC1a channels triggered by IL-6: Potential role of ASIC1a in neuroinflammation. Biochimica et biophysica acta. Molecular basis of disease, 166927.
30143647	1:1000	Lee, S.H., et al. 2018. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nature communications, 9(1), p.3434.
28318784	not listed	Murakoshi, H., et al. 2017. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron. Apr 5;94(1):37-47.e5.
26057350	not listed	Samikkannu, T., et al. 2015. HIV subtypes B and C gp120 and methamphetamine interaction: dopaminergic system implicates differential neuronal toxicity. Scientific reports, 5, p.11130.
24855644	1:3000	Coultrap, S.J., et al. 2014. Nitric oxide induces Ca2+-independent activity of the Ca2+/calmodulin-dependent protein kinase II (CaMKII). J. Biol Chem. 289(28):19458-65.
23255606	1:10,000	Magupalli, V.G., et al. 2013. Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648.

Western Blot: Mouse
PMID	Dilution	Publication
39077112	1:1000	Gissibl, T., et al. 2024. The inotropic and arrhythmogenic effects of acutely increased late INa are associated with elevated ROS but not oxidation of PKARIα. Frontiers in Cardiovascular Medicine, 1379930.
38565288	1:1000	Burch, AM, et al. 2024. TRPM2 and CaMKII Signaling Drives Excessive GABAergic Synaptic Inhibition Following Ischemia. The Journal of Neuroscience, .
37907140	1:1000	Castellanos, LCS, et al. 2023. Redistribution of ASIC1a channels triggered by IL-6: Potential role of ASIC1a in neuroinflammation. Biochimica et biophysica acta. Molecular basis of disease, 166927.
37648853	1:2500	Tullis, JE, et al. 2023. LTP induction by structural rather than enzymatic functions of CaMKII. Nature, 146-153.
34965414	1:2000	Buonarati, O.R., et al. 2021. Conserved and Divergent Features of Neuronal CaMKII Holoenzyme Structure, Function, and High-Order Assembly. Cell Reports, 110168.
34704002	1:2500	Tullis, J.E., et al. 2021. GluN2B S1303 Phosphorylation by CaMKII or DAPK1: No Indication for Involvement in Ischemia or LTP. iScience, 103214.
33881939	1:2000	Goodell, D.J., et al. 2021. Young DAPK1 knockout mice have altered presynaptic function. Journal of Neurophysiology, 125(5), pp1973-1981.
33853773	1:1000	Cook, S.G., et al. 2021. CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision. Science Advances, 7(16), p.eabe2300.
33449853	1:1000	Kreitmeier, K.G., et al. 2021. CaMKIIδ Met281/282 oxidation is not required for recovery of calcium transients during acidosis. American Journal of Physiology-Heart and Circulatory Physiology, January 15.
31914378	1:2500	Buonarati, O.R., et al. 2020. CaMKII versus DAPK1 Binding to GluN2B in Ischemic Neuronal Cell Death after Resuscitation from Cardiac Arrest. Cell Reports, 30(1), pp.1-8.
30282026	1:1000	Glasgow, S.D., et al. 2018. Activity-dependent netrin-1 secretion drives synaptic insertion of GluA1-containing AMPA receptors in the hippocampus. Cell Rep, 25(1):168-182.
27622724	not listed	Huang, L., et al. 2017. Long-lasting behavioral effects in neonatal mice with multiple exposures to ketamine-xylazine anesthesia. Neurotoxicology and Teratology.
18417701	1:10,000	Sun, X., et al. 2008. Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 - 4230.
17855343	not lited	Townsend, M., et al. 2008. Soluble A-beta Inhibits Specific Signal Transduction Cascades Common to the Insulin Receptor Pathway. J. Biol. Chem., 282: 33305 - 33312.

Western Blot: Rat
PMID	Dilution	Publication
36331254	not listed	Kazami, M., et al. 2022. Ca2+/Calmodulin induces translocation of membrane-associated TSC2 to the nucleus where it suppresses CYP24A1 expression. Bioscience, Biotechnology, and Biochemistry, zbac174.
35620430	1:2500	Brown, C.N., et al. 2022. Aβ-induced Synaptic Impairments Require CaMKII Activity That is Stimulated by Indirect Signaling Events. iScience, 104368.
34687697	1:1000	Rumian, N.L., et al. 2021. CaMKIIα knockout protects from ischemic neuronal cell death after resuscitation from cardiac arrest. Brain Research, 1773, p147699.
34667946	not listed	Brown, C.N., et al. 2021. Characterization of Six CaMKIIα Variants Found in Patients with Schizophrenia. iScience, 103184.
34544759	1:1000	Murray, C.H., et al. 2021. CaMKII Modulates Diacylglycerol Lipase-α Activity in the Rat Nucleus Accumbens after Incubation of Cocaine Craving. eNeuro, 8(5).
33444640	1:1000	Murray, C.H., et al. 2021. mGlu5 function in the nucleus accumbens core during the incubation of methamphetamine craving. Neuropharmacology, p.108452.
30143647	1:1000	Lee, S.H., et al. 2018. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nature communications, 9(1), p.3434.
28318784	not listed	Murakoshi, H., et al. 2017. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron. Apr 5;94(1):37-47.e5.
24843070	1:3000	Barcomb, K., et al. 2014. Autonomous CaMKII requires further stimulation by Ca2+/calmodulin for enhancing synaptic strength. The FASEB Journal, 28(8), 3810-3819.
24780380	1:2000	Bernard, P.B., et al. 2014. Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12.
23143515	1:1000	Shin, S.M., et al. 2012. GKAP orchestrates activity-dependent postsynaptic protein remodeling and homeostatic scaling. Nature neuroscience, 15(12), p.1655-1666.
20424167	not listed	Vest, R.S., et al. 2010. Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024-5033.
20353941	not listed	Coultrap, S.J., et al. 2010. CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin. J. Biol. Chem., 285: 17930 - 17937.
18819923	1:1000	Davies, K.D., et al. 2008. Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. J. Biol. Chem., 283: 33138 - 33146.
18417701	1:10,000	Sun, X., et al. 2008. Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 - 4230.
17942605	1:500	Sun, X., et al. 2008. Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024 - 5033.

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Anti-CaM Kinase II (Thr286) Antibody

SKU: p1005-286

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-CaM Kinase II (Thr286) Antibody

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