Our Anti-GABAB Receptor (Ser783), R2-Subunit rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAB Receptor (Ser783), R2-Subunit and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Western blot of rat synaptic membrane lysate showing specific immunolabeling of the ~102 kDa GABAB R2 protein phosphorylated at Ser783 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-B subtype of receptors. GABA-B receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABA-B receptors and phosphorylates Ser-783 in the cytoplasmic tail of the R2 subunit and that Ser-783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of Ser-783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, IHC, WB
Rabbit
GABBR2
102 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 of the R2 subunit of rat GABAB recerptor, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:500
ICC: 1:500
Unconjugated
Specific for endogenous levels of the ~102 kDa GABAB R2 phosphorylated at Ser783. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser783
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.
Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.
Marron Fernandez de Velasco, E., et al. 2015. Sex differences in GABA B R-GIRK signaling in layer 5/6 pyramidal neurons of the mouse prelimbic cortex. Neuropharmacology. Aug;95:353-60.
Kim, S.H., et al. 2020. Electroconvulsive seizures induce autophagy by activating the AMPK signaling pathway in the rat frontal cortex. International Journal of Neuropsychopharmacology, 23(1), pp.42-52.
Kim, S.H., et al. 2018. The antipsychotic agent clozapine induces autophagy via the AMPK-ULK1-Beclin1 signaling pathway in the rat frontal cortex. Progress in Neuro-Psychopharmacology and Biological Psychiatry. Feb 2;81:96-104.
Terunuma, M., et al. 2010. Prolonged activation of NMDA receptors promotes dephosphorylation and alters postendocytic sorting of GABAB receptors. Proc Natl Acad Sci USA 107: 13918-13923.
Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.
Bulk Order Anti-GABAB Receptor (Ser783), R2-Subunit Antibody
