Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody
Our Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Potassium Chloride Cotransporter (KCC2) (Ser940) and is antigen affinity purified from pooled serum. It is great for use in WB, IP.
Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Ser940 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).
Product Specific References for Applications and Species
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 29028184 | 20-100ug of antibody per 1-10mg lysate | Mahadevan, V., et al. 2017. Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition. Elife, 6, p.e28270. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 37688574 | 1:5000 | Liao, W, et al. 2023. CDKL5-mediated developmental tuning of neuronal excitability and concomitant regulation of transcriptome. Human Molecular Genetics, . |
| 36889319 | 1:1000 | Jarvis, R., et al. 2023. Direct Activation of KCC2 Arrests Benzodiazepine Refractory Status Epilepticus and Limits the Subsequent Neuronal Injury in Mice. Cell Reports Medicine, 100957. |
| 31308096 | 1:1000 | Gomez, C.D., et al. 2019. Early life inflammation increases CA1 pyramidal neuron excitability in a sex and age dependent manner through a chloride homeostasis disruption. Journal of Neuroscience, pp.2973-18. |
| 30689208 | 1:1000 | Mekhael, W., et al. 2019. Repeated anodal trans‐spinal direct current stimulation results in long‐term reduction of spasticity in mice with spinal cord injury. The Journal of physiology, Apr;597(8):2201-2223. |
| 29028184 | not listed | Mahadevan, V., et al. 2017. Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition. Elife, 6, p.e28270. |
| 28683314 | not listed | Ruinetti-Vargas, G., et al. 2017. Periadolescent Maturation of GABAergic Hyperpolarization at the Axon Initial Segment. Cell Reports, 20(1), pp.21-29. |
| 25733865 | not listed | Silayeva, L., et al. 2015. KCC2 activity is critical in limiting the onset and severity of status epilepticus. PNAS. Mar 2. pii: 201415126. |
| 24478630 | 1:1000 | Mòdol, L., et al. 2014. Differential effects on KCC2 expression and spasticity of ALS and traumatic injuries to motoneurons. Frontiers in cellular neuroscience, Jan 24; 8:7. |
| 22171026 | 1:1000 | Sarkar, J., et al. 2011. Neurosteroidogenesis is required for the physiological response to stress: role of neurosteroid-sensitive GABAA receptors. J Neurosci. 31(50):18198-210. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 36401060 | 1:1000 | Zhang, H., et al. 2022. Role Of KCC2 In The Regulation Of Brain-Derived Neurotrophic Factor On Ethanol Consumption In Rats. Molecular Neurobiology, . |
| 35352582 | 1:1000 | Gao, Y., et al. 2022. KCC2 receptor upregulation potentiates antinociceptive effect of GABAAR agonist on remifentanil-induced hyperalgesia. Molecular pain, 18, p.17448069221082880. |
| 34833037 | 1:1000 | Kalashnikov, V.E., et al. 2021. Prochlorperazine Withdraws the Delayed Onset Tonic Activity of Unloaded Rat Soleus Muscle: A Pilot Study. Life, 11(11), p.1161. |
| 33257295 | 1:1000 | Kwon, H.H., et al. 2020. Vitamin E reduces spasms caused by prenatal stress by lowering calpain expression. Epilepsy & Behavior, p.107609. |
| 29729247 | 1:1000 | Huang, Y.J., et al. 2018. Ionic plasticity and pain: the loss of descending serotonergic fibers after spinal cord injury transforms how GABA affects pain. Experimental Neurology, 306, pp.105-116. |
| 29092909 | 1:1000 | Conway, L.C., et al. 2017. N-ethylmaleimide increases KCC2 activity by modulating transporter phosphorylation. Journal of Biological Chemistry, Dec 29;292(52):21253-21263. |
| 27639636 | 1:1000 | Huang, Y.J., et al. 2016. Acute spinal cord injury (SCI) transforms how GABA affects nociceptive sensitization. Experimental Neurology, 285, pp.82-95. |
| 26090759 | 1:1000 | López-Álvarez, V. M., et al. 2015. Early increasing-intensity treadmill exercise reduces neuropathic pain by preventing nociceptor collateral sprouting and disruption of chloride cotransporters homeostasis after peripheral nerve injury. Pain. Jun 18. |
| 24861264 | 1:1000 | Mòdol, L., et al. 2014. Neonatal allopregnanolone or finasteride administration modifies hippocampal K+ Cl− co-transporter expression during early development in male rats. The Journal of steroid biochemistry and molecular biology, Sep; 143:343-7. |
| 24813295 | 1:1000 | Mòdol, L., et al. 2014. Prevention of NKCC1 phosphorylation avoids downregulation of KCC2 in central sensory pathways and reduces neuropathic pain after peripheral nerve injury. PAIN, Aug; 155(8):1577-90. |
| 21532577 | not listed | Lee, H.H., et al. 2014. NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci. Jun;14(6):736-43. |
Product Specific Protocols
- Western Blot Protocol: Download