Anti-Tyrosine Hydroxylase (Ser40) Antibody
Our Anti-Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects many mammalian, and many non-mammalian Tyrosine Hydroxylase (Ser40) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.
Product Specific References for Applications and Species
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 28637871 | 1:50 | Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 32778969 | 1:3000 | Nakashima, A., et al. 2020. NT5DC2 affects the phosphorylation of tyrosine hydroxylase regulating its catalytic activity. Journal of Neural Transmission, pp.1-10. |
| 28637871 | 1:50 | Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107. |
| 10936746 | 1:300 | Witkovsky, P, et al. 2000. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of chemical neuroanatomy, 19(2), pp.105-116. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 28637871 | 1:1000 | Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107. |
| 11677263 | not listed | Salvatore, M.F, et al. 2001. Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of neurochemistry, 79(2), pp.349-360. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 37133218 | 1:1000 | Le, VQ, et al. 2023. Extracellular H+ Ions are a Novel Signal for Tyrosine Hydroxylase Activation in Catecholaminergic Cells. ACS Chemical Neuroscience, 1774-1784. |
| 30803445 | 1:1000 | Areal, L.B., et al. 2019. Neuronal scaffolding protein spinophilin is integral for cocaine-induced behavioral sensitization and ERK1/2 activation. Molecular brain, 12(1), p.15. |
| 29782882 | 1:1000 | Salvatore, M.F., et al. 2018. Prolonged increase in ser31 tyrosine hydroxylase phosphorylation in substantia nigra following cessation of chronic methamphetamine. Neurotoxicology, 67, pp.121-128. |
| 29299922 | not listed | Bezem, M.T., et al. 2018. Stabilization of human tyrosine hydroxylase in maltodextrin nanoparticles for delivery to neuronal cells and tissue. Bioconjugate Chemistry Feb 21;29(2):493-502. |
| 27124386 | not listed | Salvatore, M.F., et al. 2016. Regulation of tyrosine hydroxylase expression and phosphorylation in dopamine transporter-deficient mice. ACS chemical neuroscience, 7(7), pp.941-951. |
| 19017804 | not listed | Meyer, D.A., et al. 2008. Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology. PNAS, 105: 18561 - 18566. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 38382359 | not listed | Yamaguchi, H, et al. 2024. Role of NT5DC2 in tyrosine hydroxylase phosphorylation based on the analysis of NT5DC2-binding proteins. Biochemical and Biophysical Research Communications, 149698. |
| 37865339 | 1:1000 | Szigetvari, PD, et al. 2023. The effects of phenylalanine and tyrosine levels on dopamine production in rat PC12 cells. Implications for treatment of phenylketonuria, tyrosinemia type 1 and comorbid neurodevelopmental disorders. Neurochemistry international, 105629. |
| 37634696 | 1:1000 | Kasanga, EA, et al. 2023. Nigral-specific increase in ser31 phosphorylation compensates for tyrosine hydroxylase protein and nigrostriatal neuron loss: Implications for delaying parkinsonian signs. Experimental Neurology, 114509. |
| 32778969 | not listed | Nakashima, A., et al. 2020. NT5DC2 affects the phosphorylation of tyrosine hydroxylase regulating its catalytic activity. Journal of Neural Transmission, pp.1-10. |
| 28637871 | 1:1000 | Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107. |
| 28637176 | 1:1000 | Salvatore, M.F., et al. 2017. Dissociation of striatal dopamine and tyrosine hydroxylase expression from aging-related motor decline: evidence from calorie restriction intervention. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, Dec 12;73(1):11-20. |
| 26777664 | 1:1000 | Baroso-Chinea, P., et al. 2016. Long-term controlled GDNF over-expression reduces dopamine transporter activity without affecting tyrosine hydroxylase expression in the mesostriatal system rat. Neurobiology of Disease, 88:44-54. |
| 24743698 | not listed | Izumi, Y, et al. 2014. Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death. Toxicological Sciences, 139(2), pp.466-478. |
| 24410633 | 1:1000 | Salvatore, M.F., et al. 2014. ser31 tyrosine hydroxylase phosphorylation parallels differences in dopamine recovery in nigrostriatal pathway following 6‐OHDA lesion. Journal of neurochemistry, 129(3), 548-558. |
| 22723669 | 1:100,000 | McCutcheon, J.E., et al. 2012. Dopamine neurons in the ventral tegmental area fire faster in adolescent rats than in adults. J Neurophysiology 108(6): 1620-1630. |
| 22458761 | 1:2000 | Calipari, E.S., et al. 2014. Methylphenidate and cocaine self‐administration produce distinct dopamine terminal alterations. Addiction biology, 19(2), pp.145-155. |
| 12124439 | 1:1500 | Jedynak, J.P., et al. 2002. Acute administration of cocaine regulates the phosphorylation of serine‐19,‐31 and‐40 in tyrosine hydroxylase. Journal of neurochemistry, 82(2), pp.382-388. |
| 11677263 | not listed | Salvatore, M.F, et al. 2001. Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of neurochemistry, 79(2), pp.349-360. |