Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody

5 Citations

Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.

β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)
TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (cat. ET-HA) fused to their N-termini. Actin (cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897
β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)
β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)
TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (cat. ET-HA) fused to their N-termini. Actin (cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897

Human, Mouse, Rat
WB
Chicken

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Data Sheet

SKU: ACT-1040

Volume: 400 µL
Price:
$295.00

Product Specific References for Applications and Species

Western Blot: Human
PMID Dilution Publication
35263584not listedTian, Songhai, S, et al. 2022. Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms. Cell reports, 110476.
31484065not listedTian, S., et al. 2019. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Reports, 2517-2526.
30481169not listedTian, S., et al. 2018. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. Plos Biology, e2006951.
Western Blot: Mouse
PMID Dilution Publication
363518971:1000Tian, S., et al. 2022. Identification Of TFPI As A Receptor Reveals Recombination-Driven Receptor Switching In Clostridioides Difficile Toxin B Variants. Nature Communications, 6786.
341452501:2000Chen, P., et al. 2021. Structural basis for CSPG4 as a receptor for TcdB and a therapeutic target in Clostridioides difficile infection. Nature Communications, 3748.

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