This affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034028) and human (NP_009005) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.
Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.
Human Coronin 1a is a 51,026 dalton protein (461 amino acids) selectively expressed in cells involved in immune surveillance throughout the body, including activated microglial cells of the CNS. It is a member of the WD40 (tryptophan-aspartate) gene family, which includes the beta subunit of the trimeric G proteins as the prototype. Its function is presumably in membrane structural reorganization accompanying antigen-presentation.
Affinity-Purified IgY
100 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
CORO1A CORO1
51 kDa
Synthetic peptide
Human, Mouse, Rat
AB_2313539
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a sequence shared between the human (NM_007074) and mouse (NM_009898) gene products. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
Coronin-1A (Coronin-like protein A) (Clipin-A) (Coronin-like protein p57) (Tryptophan aspartate-containing coat protein) (TACO)
UniProt (Immunogen Species): P31146
Bulk Order Anti-Coronin 1A Antibody
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