Our Anti-Insulin Receptor (Thr1160) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Insulin Receptor (Thr1160) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of HeLa cell lysate showing specific labeling of the ~95 kDa IR protein phosphorylated at Thr1160 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
The insulin receptor (IR) is a well-studied receptor tyrosine kinase composed of two α subunits, responsible for the extracellular insulin binding site, and two β subunits, responsible for intracellular protein kinase activity (Endemann et al., 1990, Chiu et al., 2010). The binding of insulin to the α subunits activates the intrinsic kinase activity located in the β subunits and subsequently initiates a cascade of phosphorylation events causing major conformational changes in the activation loop of the kinase domain, which lead to different biological functions (Chiu et al., 2010). It has been hypothesized that Thr-1160 phosphorylation affects or is affected by Tyr-1158/62/63 phosphorylation and that the conformation of Thr-1160 and pThr-1160 is likely to be affected by the phosphorylation status of the surrounding tyrosines.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
INSR
95 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1160 of human insulin receptor, conjugated to keyhole limpet hemocyanin (KLH).
Human
Bovine, Canine, Non-Human Primate, Mouse, Rat, Xenopus
AB_2492137
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~95 kDa Insulin receptor phosphorylated at Thr1160. Immunolabeling is completely eliminated with λ-phosphatase treatment.
Phosphorylated
Thr1160
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
CD220 antibody, HHF5 antibody, human insulin receptor antibody, Insr antibody, INSR_HUMAN antibody, Insulin receptor subunit beta antibody, IR 1 antibody, IR antibody, IR-1 antibody, IR1 antibody,
Chiu SL and Cline HL (2010). Insulin receptor signaling in the development of neuronal structure and function. Neural Dev. 5:7. PMID: 17544402
Endemann G, Yonezawa K, and Roth RA (1990). Phosphatidylinositol kinase or an associated protein is a substrate for the insulin receptor tyrosine kinase. J. Biol. Chem. 265:396-400. PMID: 16038406
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