Polyclonal Antibodies

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COS-7 cells expressing NDRG2-FLAG were subjected to immunofluorescent staining using Aves Labs chicken anti-NDRG2 antibody (visualized in green) and rabbit anti-FLAG antibody (visualized in red). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from the chicken anti-NDRG2 antibody and the anti-FLAG antibody specifically in transfected cells.Western blotting of mock or NDRG2-FLAG transfected COS-7 cell lysates and human, mouse, and rat brain homogenates (10 ug/lane) with Aves Labs chicken anti-NDRG2 antibody (0.2 ug/ml) and detected with anti-chicken HRP. Anti-NDRG2 recognizes endogenous NDRG2 in brain homogenates in addition to exogenous NDRG2 in COS-7 cells.
Aves Labs Anti-NDRG2 Antibody
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Adult mouse DRG was fixed in 4% paraformaldehyde, cryostat-sectioned, and then stained for PAP immunoreactivity (1:500 dilution), showing immunoreactive material in primary sensory neurons. Photomicrograph by Dr. Mark Zilka, Univ. of North Carolina.Adult mouse spinal cord was fixed in 4% paraformaldehyde, paraffin-embedded and sectioned, and then sections were stained for PAP immunoreactivity (1:500 dilution). Adjacent sections were co-stained for IB4 and Calcitonin Gene-Related Protein (CGRP) (other sensory neuronal markers). Photomicrograph by Dr. Mark Zilka, University of North Carolina.
Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.Co-staining of Coronin1 (COR, green, 1:1000 dilution) and Internexin (rabbit antibody, red, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida.
Mouse fibroblast cultures transfected with a recombinant fragment of the mouse prion protein (green staining). Non-infected cells show labeled nuclei (blue staining) due to treatment with Hoechst stain.

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