Streptavidin Conjugated with Peroxidase
Streptavidin is a 52-66 kDa (average 60 KDa) (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC).
Streptavidin is a 52-66 kDa (average 60 KDa) (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0.
Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band.
Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin;
Protease activity: 0.000018 units/mg
Isoelectric point: 7.0 +/- determined by Isoelectric focusing.
Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0
One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.
The conjugate (one mg of streptavidin conjugated with peroxidase) is supplied in one ml of 10 mM PO4, 200 mM NaCl, pH 7.5, BSA 10 mg/ml and 0.05% Proclin 300.
2-8 °C
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For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States