Biotinylated Goat Anti-Chicken IgY Secondary Antibody

Name: Biotinylated Goat Anti-Chicken IgY Secondary Antibody
Brand: Aves Labs
SKU: B-1005
Price: 195.0 USD
Availability: InStock

2 Citations

Our Biotinylated Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.

Species Reactivity

Chicken

Host Species

Goat

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Data Sheet

SKU: B-1005

Volume: 500 µL

Variant

Price:

$195.00

Quantity:

Product Details

Target

Chicken IgY

Format

Affinity Purified

Concentration

1 mg/mL

Clonality

Polyclonal

Isotype

IgG

Host Species

Goat

Antigen

IgY

Species Reactivity

Chicken

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

Physical State

Liquid

Production Notes

Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections,goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to biotin via a 6-carbon spacer.

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), sodium azide (0.02%, w/v) (as an anti- microbial).

Conjugate

Biotin

Specificity

Does not cross-react with mammalian IgGs

Quality Control

Antibodies were analyzed using immunoelectro-phoresis and a sandwich ELISA using HRP-labeled streptavidin. In this latter test, a 1:500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with tetramethylbenzidine as the substrate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Product Specific References for Applications

ELISA
Immunohistochemistry

ELISA
PMID	Dilution	Publication
20419103	1:500000	Mazumdar, K., et al. 2010. Visualization of transepithelial passage of the immunogenic 33-residue peptide from α-2 gliadin in gluten-sensitive macaques. PLoS One, e10228.

Immunohistochemistry
PMID	Dilution	Publication
35568187	1:200	Madalena, K.M., et al. 2022. Genetic deletion of the glucocorticoid receptor in Cx3cr1+ myeloid cells is neuroprotective and improves motor recovery after spinal cord injury. Experimental neurology, 114114.

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Biotinylated Goat Anti-Chicken IgY Secondary Antibody

SKU: B-1005

Product Details

Product Specific References for Applications

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