Fluoresceinated Goat Anti-Chicken IgY Secondary Antibody

26 Citations

Our Fluoresceinated Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.

Chicken
Goat

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Data Sheet

SKU: F-1005

Volume: 500 µL
Price:
$129.00

Product Details

Chicken IgY
Affinity Purified
1 mg/mL
Polyclonal
IgG
Goat
IgY
Chicken
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Liquid
Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to fluorescein isothiocyanate.
Phosphate-buffered saline (PBS) (pH 7.2), bovine serum albumin (0.2%, w/v), sodium azide (0.02%, w/v) (as an anti-microbial agent).
FITC
Does not cross-react with mammalian IgGs
Antibodies were analyzed using immunoelectrophoresis (see below) and immunocytochemistry. In this latter test, abundant fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1:10 dilution in PBS), incubation with chicken anti-vimentin antibodies (1:1000 dilution in PBS) and then incubation with GGHL-30F-2 (1:200 dilution in PBS).
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
Ice packs

Product Specific References for Applications

Cell Sorting
PMID Dilution Publication
312753780.16mg/mlChavez-Cortez, E.G., et al. 2019. Production and evaluation of an avian IgY immunotoxin against CD133+ for treatment of carcinogenic stem cells in malignant glioma: IgY immunotoxin for the …. Journal of Oncology, 2563092.
Flow Cytometry
PMID Dilution Publication
152108461:100Du, L., et al. 2004. Macrophage colony-stimulating factor differentially regulates low density lipoprotein and transferrin receptors. Journal of Lipid Research, 1733-1740.
Immunocytochemistry
PMID Dilution Publication
384312211:200De Bortoli, M, et al. 2024. Somatic loss-of-function PIK3R1 and activating non-hotspot PIK3CA mutations associated with Capillary Malformation with Dilated Veins (CMDV). The Journal of investigative dermatology, .
38191621not listedSalapa, H.E., et al. 2024. hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS). Nature communications, 356.
359986411:2000Hong, X., et al. 2022. Mitochondrial dynamics maintain muscle stem cell regenerative competence throughout adult life by regulating metabolism and mitophagy. Cell stem cell, 1298-1314.e10.
192138731:1000Ransford, G.A., et al. 2009. Pannexin 1 contributes to ATP release in airway epithelia. American Journal of Cellular and Molecular Biology, 525-534.
173480231:200Su, W., et al. 2007. Mice with GFAP‐targeted loss of neurofibromin demonstrate increased axonal MET expression with aging. Glia, 723-733.
15191546not listedRiedl, E., et al. 2004. Identification and characterization of an alternatively spliced isoform of mouse Langerin/CD207. Journal of Investigative Dermatology, 78-86.
151052731:500Johnson, M.A., et al. 2004. Mitochondrial localization of human PANK2 and hypotheses of secondary iron accumulation in pantothenate kinase‐associated neurodegeneration. Annals of the New York Academy of Sciences, 282-298.
112643011:400Hansen, M.R., et al. 2001. Multiple distinct signal pathways, including an autocrine neurotrophic mechanism, contribute to the survival-promoting effect of depolarization on spiral ganglion …. Journal of Neuroscience, 2256-2267.
Immunohistochemistry
PMID Dilution Publication
393439701:500Mao, H, et al. 2024. Regulation of leptin signaling and diet-induced obesity by SEL1L-HRD1 ER-associated degradation in POMC expressing neurons. Nature Communications, 8435.
384312211:200De Bortoli, M, et al. 2024. Somatic loss-of-function PIK3R1 and activating non-hotspot PIK3CA mutations associated with Capillary Malformation with Dilated Veins (CMDV). The Journal of investigative dermatology, .
368469121:200-1:1000Zuppo, D.A., et al. 2023. Foxm1 Regulates Cardiomyocyte Proliferation in Adult Zebrafish After Cardiac Injury. Development, dev201163.
35904445not listedHsieh, C., et al. 2022. Non-cell-autonomous activation of Hedgehog signaling contributes to disease progression in a mouse model of renal cystic ciliopathy. Human molecular genetics, 4228-4240.
350404351:1000Yang, K., et al. 2022. Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease. The Journal of clinical investigation, e146176.
349870161:500Zheng, J., et al. 2021. Prestin and electromotility may serve multiple roles in cochlear outer hair cells. Hearing Research, 108428.
33338666not listedKhalid, A.B., et al. 2021. GATA4 regulates mesenchymal stem cells via direct transcriptional regulation of the WNT signalosome. Bone, 115819.
323800321:500Orr, B.O., et al. 2020. Presynaptic homeostasis opposes disease progression in mouse models of ALS-like degeneration: evidence for homeostatic neuroprotection. Neuron, 95-111.
308437561:400Meyer, C.B., et al. 2019. Axonal damage in spinal cord is associated with gray matter atrophy in sensorimotor cortex in experimental autoimmune encephalomyelitis. Multiple Sclerosis Journal, 294-303.
297754791:1000Hassan, A., et al. 2018. Acute intermittent hypoxia and rehabilitative training following cervical spinal injury alters neuronal hypoxia-and plasticity-associated protein expression. PLoS One, e0197486.
237919811:1000Thomas, A.M., et al. 2013. Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury. Journal of Controlled Release, 421-429.
230071671:200Migliarini, S., et al. 2013. Lack of brain serotonin affects postnatal development and serotonergic neuronal circuitry formation. Molecular Psychiatry, 1106-1118.
223375021:200Verdier, V., et al. 2012. Aging of myelinating glial cells predominantly affects lipid metabolism and immune response pathways. Glia, 751-760.
192138731:1000Ransford, G.A., et al. 2009. Pannexin 1 contributes to ATP release in airway epithelia. American Journal of Cellular and Molecular Biology, 525-534.
170718551:1000Mueller, F.J., et al. 2006. Adhesive interactions between human neural stem cells and inflamed human vascular endothelium are mediated by integrins. Stem Cells, 2367-2372.
16682648not listedLocovei, S., et al. 2006. Pannexin 1 in erythrocytes: function without a gap. PNAS USA, 7655-7659.
1611329510ug/mlGarate, M., et al. 2005. Biochemical Characterization and Functional Studies of Acanthamoeba Mannose-Binding Protein. Infection and Immunity, 5775-5781.

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