Anti-Aquaporin 2 (Ser261) Antibody
Our Anti-Aquaporin 2 (Ser261) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser261) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, IP.
Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser261 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).
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Product Specific References for Applications and Species
- Immunocytochemistry: Rat
- Immunohistochemistry: Mouse Rat
- Immunoprecipitation: Mouse Rat
- Western Blot: Human | Mouse | Rat
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 28668390 | 1:500 | Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26;490(3):1039-1044. |
| 27760768 | 1:3000 | Mamuya, F.A., et al. 2016. ILK and Cytoskeletal Architecture: An Important Determinant of AQP2 Recycling and Subsequent Entry into the Exocytotic Pathway. American Journal of Physiology-Renal Physiology, pp.ajprenal-00336. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 18606813 | 1:2000 | Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. |
| 16985212 | 1:2000 | Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 26147297 | not listed | Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719. |
| 18606813 | 1:2000 | Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. |
| 16985212 | 1:2000 | Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 27889609 | 3 uL | Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529. |
| Immunoprecipitation: Rat | ||
| PMID | Dilution | Publication |
| 27889609 | 3 uL | Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 32529500 | not listed | Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 32529500 | not listed | Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8. |
| 29046300 | 1:3000 | Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391. |
| 27889609 | 1:500-1:1000 | Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529. |
| 27760768 | 1:3000 | Mamuya, F.A., et al. 2016. ILK and Cytoskeletal Architecture: An Important Determinant of AQP2 Recycling and Subsequent Entry into the Exocytotic Pathway. American Journal of Physiology-Renal Physiology, pp.ajprenal-00336. |
| 18606813 | 1:2000 | Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. |
| 16985212 | 1:2000 | Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 29046300 | 1:3000 | Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391. |
| 28668390 | 1:500 | Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26; 490(3):1039-1044. |
| 27889609 | 1:500-1:1000 | Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529. |
| 27534994 | not listed | Al-Butaineh, M.M, et al. 2016. Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308. |
| 27185292 | not listed | Speed, J.S, et al. 2016. In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251. |
| 26147297 | not listed | Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719. |
| 18606813 | 1:2000 | Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. |
| 16985212 | 1:2000 | Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700. |