Anti-ADAM22 (Cytoplasmic) Antibody (N46/30)

16 Citations

Our Anti-ADAM22 (cytoplasmic) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N46/30. It is KO validated, detects human, mouse, and rat ADAM22 (cytoplasmic), and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Cell-surface-expressed ADAM22 (F, magenta) and cell-surface-bound LGI1 (G, magenta) were live-labelled. After fixation and permeabilization, ADAM22 (total) was stained (cat. 75-083; green), with nuclear DNA (blue, F and upper panel in G). ADAM22 S905F is on the cell surface binds to LGI1 as the wild-type. When ADAM22 C401Y is expressed, no cell-surface-bound LGI1 is detected. White squares are total expression of ADAM22 (F) and LGI1–FLAG (lower panel in G, blue). CC-BY-4.0. PMID:37953841
Cell-surface-expressed ADAM22 (F, magenta) and cell-surface-bound LGI1 (G, magenta) were live-labelled. After fixation and permeabilization, ADAM22 (total) was stained (cat. 75-083; green), with nuclear DNA (blue, F and upper panel in G). ADAM22 S905F is on the cell surface binds to LGI1 as the wild-type. When ADAM22 C401Y is expressed, no cell-surface-bound LGI1 is detected. White squares are total expression of ADAM22 (F) and LGI1–FLAG (lower panel in G, blue). CC-BY-4.0. PMID:37953841
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Cell-surface-expressed ADAM22 (F, magenta) and cell-surface-bound LGI1 (G, magenta) were live-labelled. After fixation and permeabilization, ADAM22 (total) was stained (cat. 75-083; green), with nuclear DNA (blue, F and upper panel in G). ADAM22 S905F is on the cell surface binds to LGI1 as the wild-type. When ADAM22 C401Y is expressed, no cell-surface-bound LGI1 is detected. White squares are total expression of ADAM22 (F) and LGI1–FLAG (lower panel in G, blue). CC-BY-4.0. PMID:37953841
Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.
Immunoblot versus membranes from postnatal day 8 wild-type (wt) and ADAM22-knockout (ko) mice and probed with N46/30 (left) and N57/2 (right). Data courtesy of Dies Meijer, Erasmus University Rotterdam.
Immunoblot versus brain membranes from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].

Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet
KO Validated KO Validated

SKU: 75-083

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

ADAM22 (cytoplasmic)
ADAM22 is a member of the ADAM (a disintegrin and metalloproteinase) family and predominantly expressed in brain. ADAM 22 is non-catalytic unlike other proteins in the family as it does not contain a zinc binding domain. ADAM22 is a post synaptic neuron receptor for LGI 1 and also binds LGI4. ADAM22 serve as an integrin ligand. Mutations in ADAM22 are implicated in early infantile epileptic encephalopathy-61 and Brachydactyly type C.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N46/30
IgG2b
ICC, IHC, IP, WB
Mouse
Adam22
90 kDa
Fusion protein amino acids 757-857 (cytoplasmic region) of mouse ADAM22 (accession number Q9R1V6) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_10675128
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.13
WB: 1:500
IHC: 1:100
ICC: 1:100
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Disintegrin and metalloproteinase domain-containing protein 22 (ADAM 22)
Q9R1V6

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
37953841not listedNosková, L, et al. 2023. ADAM22 ethnic-specific variant reducing binding of membrane-associated guanylate kinases causes focal epilepsy and behavioural disorder. Brain Communications, .
Immunocytochemistry: Rat
PMID Dilution Publication
311648061:100Bonetto, G., et al. 2019. Selective Axonal Expression of the Kv1 Channel Complex in Pre-myelinated GABAergic Hippocampal Neurons. Frontiers in cellular neuroscience, 222.
305985021:100Hivert, B., et al. 2019. ADAM22 and ADAM23 modulate the targeting of the Kv1 channel-associated protein LGI1 to the axon initial segment. Journal of cell science, jcs219774.
Immunohistochemistry: Mouse
PMID Dilution Publication
38194969not listedMiyazaki, Y., et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634.
26269648not listedKole, M., et al. 2015. Selective Loss of Presynaptic Potassium Channel Clusters at the Cerebellar Basket Cell Terminal Pinceau in Adam11 Mutants Reveals Their Role in Ephaptic Control of Purkinje Cell Firing. The Journal of neuroscience : the official journal of the Society for Neuroscience, 11433-11444.
Immunohistochemistry: Rat
PMID Dilution Publication
20089912not listedOgawa, Y., et al. 2010. ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. The Journal of neuroscience : the official journal of the Society for Neuroscience, 1038-1048.
Immunoprecipitation: Mouse
PMID Dilution Publication
296701001:250Yamagata, A., et al. 2018. Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22. Nature communications, 1546.
Western Blot: Human
PMID Dilution Publication
22473675not listedDhanchak, A., et al. 2012. Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis. Annals of neurology, 601-613.
20463223not listedThomas, R., et al. 2010. LGI1 is a Nogo receptor 1 ligand that antagonizes myelin-based growth inhibition. The Journal of neuroscience : the official journal of the Society for Neuroscience, 6607-6612.
Western Blot: Mouse
PMID Dilution Publication
38194969not listedMiyazaki, Y, et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634.
34910912not listedYokoi, N., et al. 2021. 14-3-3 proteins stabilize LGI1-ADAM22 levels to regulate seizure thresholds in mice. Cell reports, 110107.
308463101:200Tai, Y., et al. 2019. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron, 358-372.
28452394not listedFrank, R., et al. 2017. Hierarchical organization and genetically separable subfamilies of PSD95 postsynaptic supercomplexes. Journal of neurochemistry, 504-511.
262696481:200Kole, M., et al. 2015. Selective Loss of Presynaptic Potassium Channel Clusters at the Cerebellar Basket Cell Terminal Pinceau in Adam11 Mutants Reveals Their Role in Ephaptic Control of Purkinje Cell Firing. The Journal of neuroscience : the official journal of the Society for Neuroscience, 11433-11444.
25707359not listedChen, N., et al. 2015. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes. Biochimica et biophysica acta, 827-833.
197012041:1000Zhou, Y., et al. 2009. Arrested maturation of excitatory synapses in autosomal dominant lateral temporal lobe epilepsy. Nature medicine, 1208-1214.

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