Anti-β-Catenin (Tyr-142) [γ-Catenin (Tyr-133)], Phosphospecific Antibody

9 Citations

Our β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)] rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)] and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of Hct116 src transformed cells (20 µg/lane) serum starved overnight or treated with pervanadate (1 mM) for 30 min. The blot was probed with anti-β-Catenin or anti-β-Catenin (Tyr-142).
Western blot analysis of Hct116 src transformed cells (20 µg/lane) serum starved overnight or treated with pervanadate (1 mM) for 30 min. The blot was probed with anti-β-Catenin or anti-β-Catenin (Tyr-142).
Roll over image to zoom in Click on image to zoom
Western blot analysis of Hct116 src transformed cells (20 µg/lane) serum starved overnight or treated with pervanadate (1 mM) for 30 min. The blot was probed with anti-β-Catenin or anti-β-Catenin (Tyr-142).
Immunocytochemical labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal β-Catenin and rabbit polyclonal β-Catenin (Tyr-142) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Human, Mouse, Rat
ICC, WB
Rabbit

Go to Full Product Details

SKU: CP1081

Volume: 100 µL
1-2 business days
Price:
$340.00

Product Details

β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)]
β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
CTNNB1
92
Phospho-β-Catenin (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human β-Catenin. This peptide sequence has one amino acid difference from a sequence around tyrosine 133 of human γ-Catenin. These human sequences are highly conserved in rat and mouse β- and γ-Catenins.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
ICC: 1:100
Unconjugated
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho-β-Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of Hct116 src-transformed cells treated with pervanadate, but not in control cells. Similar results were observed in pervanadate-treated A431 and human endothelial cells.
Phosphorylated
Tyr-142
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Catenin beta1, CTNNB1, catenin
P35222

Product Specific References for Applications and Species

ELISA: Mouse
PMID Dilution Publication
217054961:500Beard Jr, R.S., et al. 2011. Hyperhomocysteinemia increases permeability of the blood-brain barrier by NMDA receptor-dependent regulation of adherens and tight junctions. Blood, 2007-2014.
Immunocytochemistry: Drosophila
PMID Dilution Publication
258030411:200Hamada-Kawaguchi, N., et al. 2015. Btk29A-mediated tyrosine phosphorylation of armadillo/β-catenin promotes ring canal growth in Drosophila oogenesis. PLoS One, e0121484.
Immunocytochemistry: Mouse
PMID Dilution Publication
25266662not listedTsuneki, M., et al. 2014. Adhesion molecule-mediated hippo pathway modulates hemangioendothelioma cell behavior. Molecular and Cellular Biology, 4485-4499.
Immunohistochemistry: Drosophila
PMID Dilution Publication
297530161:200Kline, A, et al. 2018. The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber. Developmental biology, 99-112.
Western Blot: Human
PMID Dilution Publication
34252536not listedBoudria, R, et al. 2021. Regulatory interplay between Vav1, Syk and β-catenin occurs in lung cancer cells. Cell signalling, 110079.
24160326not listedQi, F., et al. 2013. Continuous exposure to chrysotile asbestos can cause transformation of human mesothelial cells via HMGB1 and TNF-α signaling. American Journal of Pathology, 1654-1666.
Western Blot: Mouse
PMID Dilution Publication
30747223not listedHuynh, H, et al. 2019. Sorafenib/MEK inhibitor combination inhibits tumor growth and the Wnt/β‑catenin pathway in xenograft models of hepatocellular carcinoma. International journal of oncology, 1123-1133.
Western Blot: Rat
PMID Dilution Publication
309882991:2000Wong, HLX, et al. 2019. Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling. Nature communications, 1745.
22558232not listedKrejci, P., et al. 2012. Receptor tyrosine kinases activate canonical WNT/β-catenin signaling via MAP kinase/LRP6 pathway and direct β-catenin phosphorylation. PLoS One, e35826.

Bulk Order Anti-β-Catenin (Tyr-142) [γ-Catenin (Tyr-133)], Phosphospecific Antibody

Related Products

Recently Viewed