Anti-CRMP2 (Thr-555), Phosphospecific Antibody

Name: Anti-CRMP2 (Thr-555), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP2251
Price: 340.0 USD
Availability: InStock

10 Citations

Our CRMP2 (Thr-555) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CRMP2 (Thr-555) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2165 or CX2255).

Western blot of rat PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: CP2251

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Product Details

Target

CRMP2 (Thr-555)

Target Description

CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

DPYSL2

Molecular Weight

Antigen

Phospho-CRMP2 (Thr-555) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-555 in human CRMP2. This sequence is conserved in rat and mouse CRMP2 and the phospho-site is not conserved in other CRMP family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unphosphorylated CRMP2 (Thr-555) before affinity purification using phospho-CRMP2 (Thr-555) peptide (without carrier). The antibody detects a 70 kDa* protein corresponding to CRMP2 on immunoblots of rat PC12 or mouse C2C12 cells treated with calyculin A. This reactivity can be specifically blocked using phospho-CRMP2 (Thr-555) peptide (CX2255).

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-555

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DRP-2, Toad-64, CRMP-62

UniProt ID

Q16555

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
26804001	not listed	Salvador, V.R., et al. 2016. Anti-glycan antibodies halt axon regeneration in a model of Guillain Barrè Syndrome axonal neuropathy by inducing microtubule disorganization via RhoA-ROCK-dependent inactivation of CRMP-2. Experimental Neurology, 42-53.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32860158	1:200	Winkler, SC, et al. 2020. PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells. Molecular Neurobiology, 5150-5166.

Western Blot: Human
PMID	Dilution	Publication
25147500	1:400	Sutinen, E.M., et al. 2014. Interleukin-18 alters protein expressions of neurodegenerative diseases-linked proteins in human SH-SY5Y neuron-like cells. Frontiers in Cellular Neuroscience, 214.
22431514	not listed	Yoneda, A., et al. 2012. A collapsin response mediator protein 2 isoform controls myosin II-mediated cell migration and matrix assembly by trapping ROCK II. Molecular and Cellular Biology, 1788-1804.
21943307	1:1000	Crews, L., et al. 2011. Phosphorylation of collapsin response mediator protein-2 disrupts neuronal maturation in a model of adult neurogenesis: Implications for neurodegenerative disorders. Molecular Neurodegeneration, 67.

Western Blot: Mouse
PMID	Dilution	Publication
32860158	1:500	Winkler, SC, et al. 2020. PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells. Molecular Neurobiology, 5150-5166.
29066950	1:500	Quarta, S, et al. 2017. Sphingosine-1-Phosphate and the S1P3 Receptor Initiate Neuronal Retraction via RhoA/ROCK Associated with CRMP2 Phosphorylation. Frontiers in molecular neuroscience, 317.
27940916	not listed	Dustrude, E.T., et al. 2016. Hierarchical CRMP2 posttranslational modifications control NaV1.7 function. PNAS USA, E8443-E8452.
21943307	1:1000	Crews, L., et al. 2011. Phosphorylation of collapsin response mediator protein-2 disrupts neuronal maturation in a model of adult neurogenesis: Implications for neurodegenerative disorders. Molecular Neurodegeneration, 67.

Western Blot: Rat
PMID	Dilution	Publication
31080913	not listed	Moutal, A, et al. 2019. Cdk5-mediated CRMP2 phosphorylation is necessary and sufficient for peripheral neuropathic pain. Neurobiology of pain (Cambridge, Mass.), 100022.
26804001	not listed	Salvador, V.R., et al. 2016. Anti-glycan antibodies halt axon regeneration in a model of Guillain Barrè Syndrome axonal neuropathy by inducing microtubule disorganization via RhoA-ROCK-dependent inactivation of CRMP-2. Experimental Neurology, 42-53.
25782368	not listed	François-Moutal, L, et al. 2015. A membrane-delimited N-myristoylated CRMP2 peptide aptamer inhibits CaV2.2 trafficking and reverses inflammatory and postoperative pain behaviors. Pain, 1247-1264.
22431514	not listed	Yoneda, A., et al. 2012. A collapsin response mediator protein 2 isoform controls myosin II-mediated cell migration and matrix assembly by trapping ROCK II. Molecular and Cellular Biology, 1788-1804.
21943307	1:1000	Crews, L., et al. 2011. Phosphorylation of collapsin response mediator protein-2 disrupts neuronal maturation in a model of adult neurogenesis: Implications for neurodegenerative disorders. Molecular Neurodegeneration, 67.

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Anti-CRMP2 (Thr-555), Phosphospecific Antibody

SKU: CP2251

Product Details

Product Specific References for Applications and Species

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