Anti-VGlut3 Antibody (N34/34)

Name: Anti-VGlut3 Antibody
Brand: NeuroMab
SKU: 75-073
Price: 319.0 USD
Availability: InStock

Our Anti-VGlut3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N34/34. It detects electric ray, human, mouse and rat VGlut3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Transfected cell immunoblot: extracts of CV-1 fibroblast cells transfected with VGlut3 (+) or left untransfected (-) and probed with N34/34 TC supe. Image courtesy of Jeff Erickson, Louisiana State University Health Sciences Center.

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N34/34 (left) or N52A/42 (right) TC supe.

Adult rat brain immunohistochemistry.

Species Reactivity

Drosophila, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-073

Product Details

Target

VGlut3

Target Description

Vesicular glutamate transporter 3 (VGLUT3) or Solute Carrier Family 17 Member 8 is encoded by the gene SLC17A8 and belongs to the vesicular glutamate transporter family. Vglut3 transports glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. Vglut3 is expressed in many tissues including brain, kidney and liver. In brain, it is expressed within glutamatergic neurons in the amygdala, brainstem, cerebral cortex, dorsal root ganglia, dorsal spinal cord, hippocampus, and hypothalamus. Diseases associated with SLC17A8 include Deafness.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N34/34

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Slc17a8 Vglut3

Molecular Weight

65 kDa

Antigen

Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGlut3 (accession number NP_714947) produced recombinantly in E. Coli

Species Reactivity

Drosophila, Human, Mouse, Rat

Antibody Registry ID

AB_2187711

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.6

Dilution Ranges

WB: 1:100
IHC: 1:250
ICC: 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Vesicular glutamate transporter 3 (VGluT3) (Solute carrier family 17 member 8)

UniProt Details

UniProt (Human): Q8NDX2
UniProt (Immunogen Species): Q7TSF2

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Electric Ray | Mouse | Rat
Western Blot: Electric Ray | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
32783653	1:100	Sepehrimanesh, M., et al. 2020. Generation and optimization of highly pure motor neurons from human induced pluripotent stem cells via lentiviral delivery of transcription factors. American journal of physiology. Cell physiology, c771-c780.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
23540692	1:500	Li, L., et al. 2013. Hijacking the neuronal NMDAR signaling circuit to promote tumor growth and invasion. Cell, 86-100.

Immunocytochemistry: Rat
PMID	Dilution	Publication
21211553	1:100	Fyk-Kolodziej, B., et al. 2011. Vesicular glutamate transporters: spatio-temporal plasticity following hearing loss. Neuroscience, 218-239.

Immunohistochemistry: Electric Ray
PMID	Dilution	Publication
24744885	not listed	Li, H., et al. 2014. Individual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP. Physiological reports, e00206.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
23540692	1:500	Li, L., et al. 2013. Hijacking the neuronal NMDAR signaling circuit to promote tumor growth and invasion. Cell, 86-100.

Immunohistochemistry: Rat
PMID	Dilution	Publication
31166818	1:10	Gagliuso, A., et al. 2019. Vestibular neurons with direct projections to the solitary nucleus in the rat. Journal of neurophysiology, 512-524.
27189764	1:300	Angioni, L., et al. 2016. Involvement of nigral oxytocin in locomotor activity: A behavioral, immunohistochemical and lesion study in male rats. Hormones and behavior, 23-38.
26257610	1:100	Fyk-Kolodziej, B., et al. 2015. Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma. Frontiers in neuroanatomy, 88.

Western Blot: Electric Ray
PMID	Dilution	Publication
24744885	not listed	Li, H., et al. 2014. Individual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP. Physiological reports, e00206.

Western Blot: Rat
PMID	Dilution	Publication
21211553	1:100	Fyk-Kolodziej, B., et al. 2011. Vesicular glutamate transporters: spatio-temporal plasticity following hearing loss. Neuroscience, 218-239.

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Anti-VGlut3 Antibody (N34/34)

SKU: 75-073

Product Details

Product Specific References for Applications and Species

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