phosphosolutions

Western blot analysis of control and pervanadate-treated A431 cells (20 µg/lane). Blots were probed with either rabbit polyclonal anti-N-WASP (Ser-484/Ser-485) or anti-N-WASP (Tyr-256).

Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal N-WASP/WASP (WP2101) or rabbit polyclonal N-WASP (Tyr-256) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-N-WASP (Tyr-256), Phosphospecific Antibody

$340

Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 µg/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).

PhosphoSolutions Anti-N-WASP Antibody

$340

Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 µg/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).

Immunocytochemical labeling of phospho- and unphospho-N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP (Ser-484/Ser-485) phospho-specific and N-WASP (Ser-484/Ser-485) unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of blocking peptide (WX2205 or WX2405).

PhosphoSolutions Anti-N-WASP Antibody

$340

Western blot of NM0161 immunoprecipitates (IP) and whole lysates. The NM0161 antibody only (lane 1), IP from A431 cell lysate (lane 2), A431 cell input (lane 3), LNCaP cells (lane 4), MeWo cells (lane 5), and normal human lung (lane 6) and skin (lane 7). The blot was probed with anti-Na+/K+ ATPase β3 NM0161 (lanes 1-7). The arrow designates native antibody, while the β3 subunit migrates around 40 kDa.

Immunocytochemical labeling of Na+/K+ ATPase β3 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase β3 (clone M016). The antibody was detected using goat anti-mouse Ig DyLight® 594.

PhosphoSolutions Anti-Na+/K+ ATPase β3 (Extracellular) Antibody

$370

Western blot of native human MDA-MB-231 (lane 1), and MeWo (lane 2) cell lysates, as well as native human breast (lane 3), lung (lane 4), skin (lane 5), and brain (lane 6) tissues. The blot was probed with anti-Na+/K+ ATPase β3 (NM0251) at 1:1000.

Immunocytochemical labeling of Na+/K+ ATPase β3 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase β3 (clone M025). The antibody was detected using goat anti-mouse Ig DyLight® 594.

PhosphoSolutions Anti-Na+/K+ ATPase β3 (Extracellular) Antibody

$370

Western Blot of MCF-7 whole cell lysate (15 ug) showing specific immunolabeling of NABC1.

PhosphoSolutions Anti-NABC1 Antibody

$445

PhosphoSolutions Anti-Nanog Antibody

$445

Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat. p1311-53, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)

PhosphoSolutions Anti-NCC (Thiazide Sensitive NaCl Cotransporter) (Thr53) Antibody

From $160

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.

Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.

PhosphoSolutions Anti-Nestin Antibody

From $160

PhosphoSolutions Anti-Nestin Antibody

$445

Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.

Immunostaining of human cerebellar cortex showing labeling of NF-H (cat. 1450-NFH, DAB, 1:500) in basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image and the molecular layer at the top. Section was counterstained with Hematoxylin.

PhosphoSolutions Anti-Neurofilament NF-H Antibody

From $150

Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.

PhosphoSolutions Anti-Neurofilament NF-H Antibody

From $150

Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Immunostaining of mixed cultured rat neurons stained with anti-NFL antibody (cat. 1452-NFL, green, 1:100) and anti-alpha internexin.

PhosphoSolutions Anti-Neurofilament NF-L Antibody

From $150

Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN (cat. 583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.

PhosphoSolutions Anti-Neurofilament NF-L Antibody

From $150

Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.

Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M (cat. 1454-NFM, 1:5000, red) and anti-Neurofilament-H (cat. 1451-NFH, 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.

PhosphoSolutions Anti-Neurofilament NF-M Antibody

From $150

Immunofluorescence of a section of rat cerebellum labeled with anti-Neurofilament-M (cat. 1455-NFM, 1:1000, red) and anti-CNP (green). The anti-NFM labels the network of axons of basket neurons and other neurons. The blue is DAPI staining of nuclear DNA.

PhosphoSolutions Anti-Neurofilament NF-M Antibody

From $150

Western blot image of recombinant human Neuropilin-1 (lanes 1 & 3) and human PC3 cells (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-Neuropilin-1 (NP2111) (lanes 1 & 2) or with mouse monoclonal anti-Neuropilin-1 (lanes 3 & 4).

PhosphoSolutions Anti-Neuropilin-1 (a1 CUB Domain) Antibody

$340

Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NP2111) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).

Immunocytochemical double labeling using anti-HA mouse monoclonal and anti-NRP-1 rabbit polyclonal (NP2111) antibodies in COS-7 cells mock transfected (A,D) or transfected with NRP-1 constructs (B,E). The specificity of the binding in E was demonstrated by using NRP-1 peptide (NX2115) in the presence of the anti-NRP-1 antibody (C,F).

PhosphoSolutions Anti-Neuropilin-1 (a1 CUB Domain) Antibody

$340

Western blot of 20’ UV treated HeLa lysate showing specific immunolabeling of the ~65 kDa NF-κB p65 phosphorylated at Ser316 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).

PhosphoSolutions Anti-NF-kB p65 (Ser316) Antibody

From $160

Immunostaining of a sagittal section of FFPE rat brain showing strong staining of oligodendrocyte precursor cells (OPC) in the hippocampus using our anti-NG2/CSPG4 (DAB, 1:200) and detected with anti-rabbit HRP. The section is counterstained with hematoxylin to identify DNA (blue).

Western blot of mouse whole brain lysate showing specific immunolabeling of the ~250 kDa CSPG4/NG2 protein.

PhosphoSolutions Anti-NG2/CSPG4 Antibody

From $160

Western blot of mouse habenula lysate showing specific immunolabeling of the ~52 kDa nAChRβ2 protein.

PhosphoSolutions Anti-Nicotinic Acetylcholine Receptor (nAChR) β2 Antibody

From $160

Western blot of mouse habenula lysate showing specific immunolabeling of the ~52 kDa nAChRβ4 protein.

PhosphoSolutions Anti-Nicotinic Acetylcholine Receptor (nAChR) β4 Antibody

From $160

PhosphoSolutions Anti-NKIAMRE (C-terminus) Antibody

$445

PhosphoSolutions Anti-NKIAMRE (N-terminus) Antibody

$445

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