PhosphoSolutions

Western blot of 10 µg of rat cerebellar lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor.

PhosphoSolutions Anti-NMDA NR2C Subunit Antibody

$445

Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).

Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-nNOS (C-terminal region) Antibody

$370

Formalin fixed, citric acid treated parafin sections of adult Rat striatum. Sections were probed with anti-nNOS (NP2141) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

PhosphoSolutions Anti-nNOS (C-terminal region) Antibody

$285

PhosphoSolutions Anti-nNOS Antibody

$445

Western Blot of 20 ug of human hippocampus lysate (lanes 2 and 4) showing specific immunolabeling of Noggin and peptide absorption control in lane 3.

PhosphoSolutions Anti-Noggin Antibody

$445

Western Blot of Lane 1: NIH3T3 cells. Lane 2: NIH3T3 cells transfected with NOL3. Lane 3: Recombinant ARC. Lane 4: Recombinant NOL3 showing specific immunolabeling of ARC/NOL3.

PhosphoSolutions Anti-Nop30 Antibody

$445

Immunohistochemistry of 10 micron cryostat sections of rat brain fixed with 4% formaldehyde by transcardial perfusion showing specific staining of Notch 3.

PhosphoSolutions Anti-Notch 3 Homolog Antibody

$445

Western Blot of human brain lysate showing specific immunolabeling of the NPFF1 Receptor.

PhosphoSolutions Anti-NPFF1 Antibody

$445

Western Blot of brain lysate showing specific immunolabeling of NPFFR2.

PhosphoSolutions Anti-NPFF2 Antibody

$445

Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.

Immunolabeling of HEK 293 cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-UCHL1 antibody (cat. 2060-UCHL1, green, 1:500).

PhosphoSolutions Anti-NSE (Neuron Specific Enolase) Antibody

From $150

PhosphoSolutions Anti-NSE2 Antibody

$445

Western blot of rat striatal lysate showing specific labeling of the ~75 kDa NSF protein.

PhosphoSolutions Anti-NSF (N-ethylmaleimide Sensitive Fusion Protein) Antibody

From $160

Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).

Immunocytochemical labeling of nSMase2 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-nSMase2 (SP4061) antibody in the absence (Left) or presence (Right) of blocking peptide (SX4065). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.

PhosphoSolutions Anti-nSMase2 (C-terminal region) Antibody

$340

Western blot of nSMase3a and nSMase3b GFP transfectants (A) and nSMase3 siRNA knockdown in C2C12 myotubes (B). Both blots were probed with anti-nSMase3 (C-terminal region) rabbit polyclonal antibody (SP0281). (Image provided by Dr. Jennifer Moylan, Dept. Physiology, University of Kentucky).

Immunocytochemical labeling of nSMase3 in aldehyde-fixed and NP-40-permeabilized mouse C2C12 cells. The cells were labeled with rabbit polyclonal anti-nSMase3 (SP0281) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-nSMase3 (C-terminal region) Antibody

$340

Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody (cat. 1515-NPC, green, 1:100) and rabbit anti-vimentin antibody (cat. 2105-VIM, red, 1:500).

Immunolabeling of HeLa cells stained with anti-nuclear pore complex antibody (cat. 1515-NPC, red, 1:100) and rabbit anti-vimentin antibody (cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA. The anti-nuclear pore complex antibody reveals strong granular labeling of the nuclei corresponding to the protein. The anti-vimentin antibody specifically labels intermediate filaments.

PhosphoSolutions Anti-Nuclear Pore Complex Antibody

From $150

Immunostaining of HeLa cells showing specific labeling of nuclei using the anti-nuclei antibody (cat. 1590-NUC, 1:100, green).

PhosphoSolutions Anti-Nuclei Antibody

From $150

Immunocytochemical labeling of Nucleoporin p62 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with mouse monoclonal anti-Nucleoporin p62 (N-terminal region) and the antibody was detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 488.

Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate
filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).

PhosphoSolutions Anti-Nucleoporin p62 (N-terminal region) Antibody

$370

Immunohistochemistry of adult rat brain hippocampus showing specific staining of Nurr1. Tissue was fixed in paraformaldehyde and cut into 20 micron sections.

Western Blot of 20 ug of human hippocampus tissue lysate (lanes 2 and 4) showing specific immunolabeling of Nurr1. Peptide absorption control lane 3.

PhosphoSolutions Anti-Nurr1 Antibody

$445

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 27 kDa Olig1 protein.

Immunostaining of primary rat oligodendrocytes using anti-Olig1 (cat. 1537-OLIG1, red, 1:100).

PhosphoSolutions Anti-Olig1 Antibody

From $160

Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~32 kDa form of the Olig2 protein phosphorylated at Ser10,13,14 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding non-phosphopeptide (not shown).

PhosphoSolutions Anti-Olig2 (Ser10/13/14) Antibody

From $160

Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 (cat. 1538-OLIG2, red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~32 kDa Olig2 protein.

PhosphoSolutions Anti-Olig2 Antibody

From $160

Western blot analysis of human recombinant osteopontin protein (lane 1 & 2) or human MDA-MB-231 cells (lane 3 & 4). The blots were probed with mouse monoclonal anti-Osteopontin (OM5741) at a dilution of 1:1000 (lane 1), 1:4000 (lane 2), 1:250 (lane 3), and 1:1000 (lane 4).

Immunocytochemical labeling of Osteopontin in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-Osteopontin (M574). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-Osteopontin (N-terminal region) Antibody

$340

Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-P-Cadherin at 1:250 (lane 1) and 1:500 (lane 2), 1:1000 (lane 3) and 1:2000 (lane 4).

PhosphoSolutions Anti-P-Cadherin (N-terminal region) Antibody

$285

Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4) or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).

Immunocytochemical labeling of p130 Cas in aldehyde fixed human A431 epidermoid carcinoma cells. The cells were labeled with mouse monoclonal anti-p130 Cas (PM1441). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-p130 Cas (C-terminal region) Antibody

$370

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