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Western blot of 10 µg of rat cerebellar lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor.
Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).Formalin fixed, citric acid treated parafin sections of adult Rat striatum. Sections were probed with anti-nNOS (NP2141) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Western Blot of 20 ug of human hippocampus lysate (lanes 2 and 4) showing specific immunolabeling of Noggin and peptide absorption control in lane 3.
Western Blot of Lane 1: NIH3T3 cells. Lane 2: NIH3T3 cells transfected with NOL3. Lane 3: Recombinant ARC. Lane 4: Recombinant NOL3 showing specific immunolabeling of ARC/NOL3.
Immunohistochemistry of 10 micron cryostat sections of rat brain fixed with 4% formaldehyde by transcardial perfusion showing specific staining of Notch 3.
Western Blot of human brain lysate showing specific immunolabeling of the NPFF1 Receptor.
Western Blot of brain lysate showing specific immunolabeling of NPFFR2.
Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).Immunocytochemical labeling of nSMase2 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-nSMase2 (SP4061) antibody in the absence (Left) or presence (Right) of blocking peptide (SX4065). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.
Western blot of nSMase3a and nSMase3b GFP transfectants (A) and nSMase3 siRNA knockdown in C2C12 myotubes (B). Both blots were probed with anti-nSMase3 (C-terminal region) rabbit polyclonal antibody (SP0281). (Image provided by Dr. Jennifer Moylan, Dept. Physiology, University of Kentucky).Immunocytochemical labeling of nSMase3 in aldehyde-fixed and NP-40-permeabilized mouse C2C12 cells. The cells were labeled with rabbit polyclonal anti-nSMase3 (SP0281) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Immunohistochemistry of adult rat brain hippocampus showing specific staining of Nurr1.  Tissue was fixed in paraformaldehyde and cut into 20 micron sections.Western Blot of 20 ug of human hippocampus tissue lysate (lanes 2 and 4) showing specific immunolabeling of Nurr1. Peptide absorption control lane 3.

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