PhosphoSolutions

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Western Blot of 50ug of human heart lysate showing specific immunolabeling of PRG3.
Western blot of GST recombinant human full-length prion protein that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 min (lanes 6 & 8). The blots were probed with anti-Prion protein (3F4) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser-43) (lanes 3, 4, 7, & 8).
Western Blot of metastatic adenocarcinoma LNCaP.FGC cell lysate showing specific immunolabeling of Prostein.
Western Blot of androgen stimulated metastatic adenocarcinoma LNCaP.FGC cells showing specific immunolabeling of RDH11.
Immunocytochemistry of fibroblasts and oral squamous carcinoma cells showing specific immunolableing of Patched 1/PTCH Antibody in upper right.  Magnification: 400x. Image from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.Immunohistochemistry showing specific staining of PTCH1 and increased protein levels throughout the growth plate in Smad2 and Smad3 mutants, particularly in the resting zone. All images are P0 proximal tibias. Image from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006352) licensed under a CC-BY license.
Western Blot of human fetal brain lysate showing specific immunolabeling of PTCH2.
Western blot image of human Jurkat cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with calyculin A (100 nM for 30 min.). The blots were probed with mouse monoclonal anti-PTP1B (lanes 1 & 2) or rabbit polyclonal anti-PTP1B (Ser-50) (lanes 3 & 4).Immunocytochemical labeling of PTP1B in methanol and acetone fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monclonal anti-PTP1B (PM2341) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B (Tyr-152) (lanes 3 & 4).Immunocytochemical labeling of PTP1B in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-PTP1B (PP2351) antibody. The antibody was detected using appropriate secondary antibody
conjugated to DyLight® 594.

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