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Immunostaining of Chinese Hamster Overy (CHO) cells stably transfected with KV3.1b gene with the phospho-Ser503 Kv3.1 subunit antibody (cat. p1550-503, green, 1:400). The image on the right shows cells that have been treated with the protein kinase C activator PMA (500nM) while the control cells are on the left.
PhosphoSolutions Anti-Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) Antibody
From $160
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Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Ser940 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) Antibody
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Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody
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PhosphoSolutions Anti-PRAC Antibody
$445
Western blot of Drosophila lysate showing specific immunolabeling of the ~60 kDa PRAS40 protein phosphorylated at Thr356 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-PRAS40 (Thr356) Antibody
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Immunofluorescence of healthy human kidney. The antibody specifically labels pre-pro-vasopressin (white) in the collecting ducts (DBA, red), but not in the proximal tubules (LTL, green) of human kidney. DNA is labeled with DAPI (blue). Image kindly provided by JP Arroyo, Vanderbilt University.Western blot of mouse whole brain lysate showing specific immunolabeling of the ~20 kDa uncleaved pre-pro vasopressin protein.
PhosphoSolutions Anti-Pre-Pro-Vasopressin Antibody
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Western Blot of 50ug of human heart lysate showing specific immunolabeling of PRG3.
PhosphoSolutions Anti-PRG3 Antibody
$445
Western blot of GST recombinant human full-length prion protein that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 min (lanes 6 & 8). The blots were probed with anti-Prion protein (3F4) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser-43) (lanes 3, 4, 7, & 8).
PhosphoSolutions Anti-Prion Protein (Ser-43), Phosphospecific Antibody
$340
Western blot of GST recombinant human full-length prion protein that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 min (lanes 6 & 8). The blots were probed with anti-Prion protein (3F4) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser-43) (lanes 3, 4, 7, & 8).
PhosphoSolutions Anti-Prion Protein Antibody
$285
Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138; PX4825) (lanes 2 & 5) or phospho-Profilin (Ser-138; PX4795) (lanes 3 & 6) blocking peptides.Immunocytochemical labeling of Profilin in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Profilin (PP4821) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-Profilin (C-terminal region) Antibody
$285
Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.Immunocytochemical labeling of Profilin in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Profilin (PP4801) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-Profilin (C-terminal region) Antibody
$285
Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138; PX4825) (lanes 2 & 5) or phospho-Profilin (Ser-138; PX4795) (lanes 3 & 6) blocking peptides.Western blot of human recombinant Profilin-1 phosphorylated in vitro with PKCα kinase then untreated (-) or treated with lambda phosphatase (+). The blots were probed with anti-Profilin (a.a. 126-137) (left panel) or anti-Profilin (Ser-138) phospho-specific (right panel) antibodies at 1:1000.
PhosphoSolutions Anti-Profilin (Ser-138), Phosphospecific Antibody
$340
Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.
PhosphoSolutions Anti-Profilin (Tyr-129), Phosphospecific Antibody
$340
Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).
PhosphoSolutions Anti-Progesterone Receptor (Ser190) Antibody
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Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).
PhosphoSolutions Anti-Progesterone Receptor (Ser294) Antibody
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Western Blot of metastatic adenocarcinoma LNCaP.FGC cell lysate showing specific immunolabeling of Prostein.
PhosphoSolutions Anti-Prostein Antibody
$445
Immunofluorescence of mouse lung showing specific labeling of alveolar type 2 epithelial cells using anti-Prosurfactant Protein C antibody in red. Nuclei are stained blue.
PhosphoSolutions Anti-Prosurfactant Protein C Antibody
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Immunostaining of rat dentate gyrus showing specific immunolabeling of the Prox1 protein(DAB, 1:1000). Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College.
PhosphoSolutions Anti-Prox1 Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~95 kDa PSD-95 protein.
PhosphoSolutions Anti-PSD-95 Antibody
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Western Blot of androgen stimulated metastatic adenocarcinoma LNCaP.FGC cells showing specific immunolabeling of RDH11.
PhosphoSolutions Anti-PSDR1 (RDH11) Antibody
$445
Immunocytochemistry of fibroblasts and oral squamous carcinoma cells showing specific immunolableing of Patched 1/PTCH Antibody in upper right. Magnification: 400x. Image from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.Immunohistochemistry showing specific staining of PTCH1 and increased protein levels throughout the growth plate in Smad2 and Smad3 mutants, particularly in the resting zone. All images are P0 proximal tibias. Image from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006352) licensed under a CC-BY license.
PhosphoSolutions Anti-PTCH1 Antibody
$445
Western Blot of human fetal brain lysate showing specific immunolabeling of PTCH2.
PhosphoSolutions Anti-PTCH2 Antibody
$445
Western blot image of human Jurkat cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with calyculin A (100 nM for 30 min.). The blots were probed with mouse monoclonal anti-PTP1B (lanes 1 & 2) or rabbit polyclonal anti-PTP1B (Ser-50) (lanes 3 & 4).Immunocytochemical labeling of PTP1B in methanol and acetone fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monclonal anti-PTP1B (PM2341) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-PTP1B (C-terminal region) Antibody
$370
Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B (Tyr-152) (lanes 3 & 4).Immunocytochemical labeling of PTP1B in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-PTP1B (PP2351) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-PTP1B (N-terminal region) Antibody
$340

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