PhosphoSolutions

Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with HSP60 as a loading control.

Immunofluorescence of SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in red. Nuclei are visualized in blue with DAPI.

PhosphoSolutions Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody

From $160

PhosphoSolutions Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody

From $160

Western blot analysis of adult mouse brain. The blot was probed with rabbit polyclonal anti-SCAI (N-terminal region) antibody in the presence (lanes 2) or absence (lane 1) of SCAI (N-terminal region) blocking peptide (SX3845), or unrelated peptide (lane 3).

PhosphoSolutions Anti-SCAI (N-terminal region) Antibody

$285

Western blot image of human A431 cells and (lane 1) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lane 2). The blots were probed with rabbit polyclonal anti-Semaphorin 3A (SP1241) at a dilution of 1:1000.

PhosphoSolutions Anti-Semaphorin-3A (C-terminal) Antibody

$340

Western blots of human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 1-4). The blots were probed with rabbit polyclonal Sema3A (Central region) at 1:250 (lane 1) and 1:1000 (lane 2) and mouse monoclonal Sema3A (Central region) at 1:250 (lane 3) and 1:1000 (lane 4). Both antibodies recognize the 95 kDa and 125 kDa forms of the recombinant Sema3A.

PhosphoSolutions Anti-Semaphorin-3A (Central region) Antibody

$340

Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.

Formalin fixed, citric acid treated parafin sections of adult Rat cerebellum. Sections were probed with anti-Sema3A (SP1221) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

PhosphoSolutions Anti-Semaphorin-3A (Central region) Antibody

$340

Immunocytochemical labeling of Sema-3A in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal Sema-3A (N-terminal) antibody (SP1401), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-Semaphorin-3A (N-terminal) Antibody

$340

Western blot analysis of rat PC12 cells (lanes 1 & 2) and human PC-3 cells (lanes 3 & 4). The blots were probed with anti-Sema-3E (N-terminal region) antibody in absence (lanes 1 & 3) or presence of Sema-3E (N-terminal region) blocking peptide (lanes 2 & 4).

PhosphoSolutions Anti-Semaphorin-3E (N-terminal region) Antibody

$285

Western blot analysis of Sema-3F expression in adult mouse brain (lane 1), kidney (lane 2), and liver (lane 3). The blot was probed with anti-Sema-3F (SP3921) at 1:1000.

PhosphoSolutions Anti-Semaphorin-3F (C-terminal region) Antibody

$285

Western blot image of mouse brain lysate. The blot was probed with mouse monoclonal Semaphorin-4D antibody (SM1881) at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

Immunocytochemical labeling in rat PC12 cells differentiated with NGF. The cells were probed with rabbit polyclonal Plexin B1 (PP1841) and mouse monoclonal Sema-4D (SM1881) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-Semaphorin-4D (C-terminal region) Antibody

$370

Western blot of Sema-6A in human colorectal cancer (CRC) cells treated with control (shC) or Sema-6A (shSema6A) shRNAs. Sema-6A was immunoprecipitated from each of the CRC lysates using Sema-6A (a.a. 772-787) or Sema-6A (C-terminus) antibody, then the blotted immunoprecipitations were probed with Sema-6A antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, Univ. of Torino, Italy).

mmunocytochemical labeling of Sema-6A in COS7 cells that were mock transfected (top images) or Sema-6A transfected (bottom images). The cells were labeled with anti-Sema-6A (a.a. 772-787) (Left top and bottom image) or anti-Sema-6A (C-terminus) (Right top and bottom image). The antibodies were detected using anti-rabbit fluorescent secondary antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, University of Torino, Italy).

PhosphoSolutions Anti-Semaphorin-6A (C-terminus) Antibody

$340

Immunocytochemical labeling of Sema-6A in COS7 cells that were mock transfected (top images) or Sema-6A transfected (bottom images). The cells were labeled with anti-Sema-6A (a.a. 772-787) (Left top and bottom image) or anti-Sema-6A (C-terminus) (Right top and bottom image). The antibodies were detected using anti-rabbit fluorescent secondary antibody. (Images provided by Dr. Luca Tamagnone from the IRCC, University of Torino, Italy).

PhosphoSolutions Anti-Semaphorin-6A (cytoplasmic region) Antibody

$340

Western blot of rat mid brain membrane lysate showing specific immunolabeling of the ~76 kDa SERT protein phosphorylated at Thr276 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

PhosphoSolutions Anti-Serotonin Transporter (Thr276) Antibody

From $160

Western Blot of 3 ug of recombinant SERPINB12 showing specific immunolabeling of SERPINB12.

PhosphoSolutions Anti-SERPINB12 Antibody

$445

Western Blot of 5 ug of normal oral mucosa lysate showing specific immunolabeling of SERPINB13.

PhosphoSolutions Anti-SERPINB13 Antibody

$445

Western Blot of human pancreas lysate showing specific immunolabeling of SFRP5.

PhosphoSolutions Anti-SFRP5 Antibody

$445

Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-Shank1 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

PhosphoSolutions Anti-Shank1 (C-terminal region) Antibody

$285

Immunohistochemistry of retinal tissue showing specific staining of Shank at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.

Immunocytochemistry of hippocampal neurons DIV 14 grown under control, ASD1, and ASD2 conditions. The fluorescence intensity of Shank positive puncta was measured using antibodies specific for Shank1, Shank2, and Shank3. Exemplary images (upper panel) and quantification of average puncta signal intensity of 10 cells per condition (lower panel). Merged images show additional DAPI staining of the nucleus. Neurons grown under ASD1 conditions show a significant decrease of synaptic Shank proteins, while neurons

PhosphoSolutions Anti-SHANK1a (C-terminus) Antibody

$445

Western Blot of rat brain lysate showing specific immunolabeling of SHANK1.

PhosphoSolutions Anti-SHANK1a (N-terminus) Antibody

$445

Western blot analysis of ShcA expression in A431 cell lysate (lanes 1, 2, 3, & 4). The blots were probed with rabbit polyclonal anti-ShcA (SP1331) at 1:1000 (lane 1) or 1:4000 (lane 2) and mouse monoclonal ShcA (C-terminal region) at 1:1000 (lane 3) or 1:4000 (lane 4).

Immunocytochemical labeling of ShcA in paraformaldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal (top) and rabbit polyclonal (bottom) ShcA antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

PhosphoSolutions Anti-ShcA Antibody

$285

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti- SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho- serine peptide (lane 6).

PhosphoSolutions Anti-SHP1 (C-terminal region) Antibody

$285

PhosphoSolutions Anti-SHP1 (Ser-591), Phosphospecific Antibody

$340

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to alkaline phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Tyr-536) antibody (lanes 3-5). The SHP1 (Tyr-536) antibody was used in the presence of phospho-SHP1 (Tyr-536) peptide (lane 5).

PhosphoSolutions Anti-SHP1 (Tyr-536), Phosphospecific Antibody

$340

Western blot analysis of adult mouse brain. The blot was probed with anti-SHP2 (N-terminal) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).

PhosphoSolutions Anti-SHP2 (N-terminal region) Antibody

$285

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