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Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).
Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diaphragm tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (AP2041; lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide (AX2045).Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 (AP2041) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-B-Raf (N-terminus) (lanes 1 & 2) or anti-B-Raf (Ser-446) (lanes 3 & 4).
Western Blot of transfected 293 cell lysate showing specific immunolabeling of Bcl10.
Western blot of 293 cells mock transfected (lane 1) or transiently transfected with pLenti6/TR lentiviral vector (lanes 2 & 3). Blots were probed with anti-Bsd (lanes 1-3). Molecular weight (MW) standards show that Bsd is expressed as a 13 kDa band in only the transfected cells.Immunocytochemical labeling of Blasticidin S Deaminase (Bsd) in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were labeled with anti-Bsd (BP1231) and detected using appropriate secondary antibody conjugated to Texas Red. (Images provided by Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Center).
Western Blot of MCF-7 whole cell lysate (15 ug) showing specific immunolabeling of BRCAA1
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).
Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-c-Cbl (CM1591; lanes 1 & 2) or anti-c-Cbl (Tyr-700) (CM1611; lanes 3 & 4).

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