neuromab

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Immunostaining of a 7 µm thick DRG neuron section from a 3 month old rat labeling NaV1.8 (green, 1:2000, Cat. 75-166), IB4 (red), and nuclei is stained with DAPI (blue). The image is kindly provided by Cristian Acosta, Universidad Nacional de Cuyo, Mendoza, Argentina.Stable/transfected cell immunoblot: extracts of HEK cells stably expressing Nav1.7 and COS cells transiently transfected with GFP-tagged Nav1.8 or untagged Kv2.1 plasmids and probed with N134/12 TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblot against crude membranes from adult rat brain (RBM) and wild-type (WT) and Navbeta2 knockout (KO) mouse brains probed with N395/68 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Lori Isom (University of Michigan).
Transfected cell immunoblotExtracts of COS cells transiently transfected with GFP-tagged Navbeta1, V5/His-tagged Navbeta2, V5/His-tagged Navbeta3, GFP-tagged Navbeta4 or untagged Kv2.1 plasmid and probed with mouse anti-V5 (center), N396/29 (left) and N52A/42 (right) TC supes.
Adult rat sciatic nerve immuno-fluorescence staining: paranodes labeled with Caspr (green) surround nodes of Ranvier labeled with Beta-IV spectrin (blue) and N168/6 (red). Images courtesy of Shelly Buffington and Matt Rasband (Baylor College of Medicine).Adult rat brain immunohistochemistry N168/6 staining in striatal efferents in globus pallidus.

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