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Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cortex layer 2/3 with L115/13 (green), GABA (red) and DAPI (blue). Volume reconstruction of 35 serial 70 nm sections show two NPY-positive neurons with varying levels of GABA reactivity, as well as some NPY-reactive axonal varicosities. Images courtesy of Kristina Micheva (Stanford).Adult rat cortex immunohistochemistry.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2, NGL-3 or untagged Kv2.1 plasmid and probed with N49A/21 (left and middle) or K89/34 (right) TC supe.
Adult mouse brain immunoblot: extracts of brains from wild-type (1), Netrin-G2 knockout (2) and NGL-2 knockout (3) mice were probed with Netrin-G2 antibody (left) or N50/36 (right). Reproduced with permission from 2007 Zhang et al Genes Brain Behav.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2, NGL-3 or untagged Kv2.1 plasmid and probed with N50/36 (left) or K89/41 (right) TC supe.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2 NGL-3 or untagged Kv2.1 plasmid and probed with N51/6 (left) or K89/41 (right) TC supe.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N161/20 TC supe (left) and a mouse loading control (right).
Adult rat cortex hippocampus immunohistochemistry.Adult rat cortex immunohistochemistry.
Array tomography immunofluorescence volume reconstruction of 9 serial LRWhite-embedded 70 nm sections from adult mouse cortex layer 6 with L121/25 (green), rabbit Synapsin (Cell Signaling #5297, red). Image courtesy of Kristina Micheva (Stanford).Array tomography immunofluorescence volume reconstruction of 9 serial LRWhite-embedded 70 nm sections from adult mouse cortex layer 6 with L121/25 (green), DAPI (blue). Image courtesy of Kristina Micheva (Stanford).

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