Anti-6xHis Antibody (N144/14)

Our Anti-6xHis mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N144/14. It detects 6xHis, and is purified by Protein A chromatography. It is great for use in ELISA, ICC, WB.



SKU: 75-169

Volume: 100 µL
Price:
Sale price$329.00
Ships: 1-2 business days

Product Details

6xHis
6xHIS or His-tag is a commonly used recombinant tag that consists of 6 sequential histidine residues placed at the amino or carboxy terminus of a targeted sequence. 6xHis is useful for purification of proteins or visualization by western blot or immunocytochemistry.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N144/14
IgG1
ELISA, ICC, IHC, WB
Mouse
N/A
1 kDa
Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.
Rat
AB_10673446
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.96
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry
PMID Dilution Publication
366531911:3Petrosyan, H.A., et al. 2023. AAV Vector Mediated Delivery of NG2 Function Neutralizing Antibody and Neurotrophin NT-3 Improves Synaptic Transmission, Locomotion, and Urinary Tract Function after Contusion Spinal Cord Contusion Injury in Adult Rats. The Journal of Neuroscience, 1492-1508.
31065282not listedLi, S., et al. 2019. Structural and Mechanistic Bases of Nuclear Calcium Signaling in Human Pluripotent Stem Cell-Derived Ventricular Cardiomyocytes. Stem Cells International, 8765752.
Immunohistochemistry
PMID Dilution Publication
341586181:200Seigneur, E., et al. 2021. Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors. Molecular Psychiatry, pp.1-13.. Molecular Psychiatry, 1-13.
Immunoprecipitation
PMID Dilution Publication
23022559not listedBrittain, J.M., et al. 2012. Cdk5-mediated phosphorylation of CRMP-2 enhances its interaction with CaV2.2. FEBS Letter, 3813-3818.
Western Blot
PMID Dilution Publication
37833406not listedMa, D, et al. 2023. A cytoskeleton-membrane interaction conserved in fast-spiking neurons controls movement, emotion, and memory. Molecular psychiatry, .
366531931:500Maor, G., et al. 2023. α-Synuclein Promotes Neuronal Dysfunction and Death by Disrupting the Binding of Ankyrin to β-Spectrin. Journal of Neuroscience, 1614-1626.
36653191not listedPetrosyan, H.A., et al. 2023. AAV Vector Mediated Delivery of NG2 Function Neutralizing Antibody and Neurotrophin NT-3 Improves Synaptic Transmission, Locomotion, and Urinary Tract Function after Contusion Spinal Cord Contusion Injury in Adult Rats. The Journal of Neuroscience, 1492-1508.
338313861:1000Simmons, Z.R., et al. 2021. Generation and characterization of a laforin nanobody inhibitor. Clinical Biochemistry, 80-89.
29425510not listedZheng, W., et al. 2018. Direct Binding between Pre-S1 and TRP-like Domains in TRPP Channels Mediates Gating and Functional Regulation by PIP2. Cell Reports, 1560-1573.
291971411:1000Aaron, P., et al. 2018. The blood-brain barrier internalises Cryptococcus neoformans via the EphA2-tyrosine kinase receptor. Cellular Microbiology, .
287137811:1000Na Pombejra, S., et al. 2017. The Metalloprotease, Mpr1, Engages AnnexinA2 to Promote the Transcytosis of Fungal Cells across the Blood-Brain Barrier. Frontiers in Cellular and Infection Microbiology, 296.
277871951:1000Cooper, S., et al. 2016. Structural determinants of adhesion by Protocadherin-19 and implications for its role in epilepsy. Elife, e18529.
24915085not listedErickson, A.I., et al. 2014. Expression, purification and preliminary crystallographic analysis of Mycobacterium tuberculosis CysQ, a phosphatase involved in sulfur metabolism.. Acta Crystallographica. Section F, Structural Biology Communications, 750-753.
24412576not listedBarry, J., et al. 2014. Ankyrin-G directly binds to kinesin-1 to transport voltage-gated Na+ channels into axons.. Developmental Cell, 117-31.
23487040not listedBarry, J., et al. 2013. Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels.. Journal of Cell Science, 2027-2041.
22940628not listedSun, Z., et al. 2012. The Gβ3 splice variant associated with the C825T gene polymorphism is an unstable and functionally inactive protein.. Cellular Signalling, 2349-2359.
22915120not listedLi, Y., et al. 2012. Molecular and functional interaction between protocadherin-γC5 and GABAA receptors.. Journal of Neuroscience, 11780-11797.
221841981:1000Emond, M.R., et al. 2011. A complex of Protocadherin-19 and N-cadherin mediates a novel mechanism of cell adhesion.. The Journal of Biology, 1115-1121.
19754155not listedHsu, S., et al. 2009. Structural insights into glucan phosphatase dynamics using amide hydrogen-deuterium exchange mass spectrometry.. Biochemistry, 9891-9902.
Unspecified
PMID Publication
31820926Aaron, P., et al. 2020. Harnessing the Activity of the Fungal Metalloprotease, Mpr1, To Promote Crossing of Nanocarriers through the Blood-Brain Barrier. ACS Infectious Diseases, 138-149.
25485908Yokoi, N., et al. 2015. Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy.. Nature Medicine, 19-26.
24240280Benoit, R.M., et al. 2014. Structural basis for recognition of synaptic vesicle protein 2C by botulinum neurotoxin A.. Nature, 108-111.

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