Anti-ADAM22 (Cytoplasmic) Antibody (N46/30)
Our Anti-ADAM22 (cytoplasmic) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N46/30. It is KO validated, detects human, mouse, and rat ADAM22 (cytoplasmic), and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblot versus brain membranes from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-083-n46-30-wb-3_{width}x.jpg?v=1704383960)
Cell-surface-expressed ADAM22 (F, magenta) and cell-surface-bound LGI1 (G, magenta) were live-labelled. After fixation and permeabilization, ADAM22 (total) was stained (cat. 75-083; green), with nuclear DNA (blue, F and upper panel in G). ADAM22 S905F is on the cell surface binds to LGI1 as the wild-type. When ADAM22 C401Y is expressed, no cell-surface-bound LGI1 is detected. White squares are total expression of ADAM22 (F) and LGI1–FLAG (lower panel in G, blue). CC-BY-4.0. PMID:37953841
![Immunoblot versus brain membranes from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-083-n46-30-wb-3_1024x.jpg?v=1704383960)
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Rat
- Immunohistochemistry: Mouse | Rat
- Immunoprecipitation: Mouse
- Western Blot: Human | Mouse
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 37953841 | not listed | Nosková, L, et al. 2023. ADAM22 ethnic-specific variant reducing binding of membrane-associated guanylate kinases causes focal epilepsy and behavioural disorder. Brain Communications, . |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 31164806 | 1:100 | Bonetto, G., et al. 2019. Selective Axonal Expression of the Kv1 Channel Complex in Pre-myelinated GABAergic Hippocampal Neurons. Frontiers in cellular neuroscience, 222. |
| 30598502 | 1:100 | Hivert, B., et al. 2019. ADAM22 and ADAM23 modulate the targeting of the Kv1 channel-associated protein LGI1 to the axon initial segment. Journal of cell science, jcs219774. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38194969 | not listed | Miyazaki, Y., et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634. |
| 26269648 | not listed | Kole, M., et al. 2015. Selective Loss of Presynaptic Potassium Channel Clusters at the Cerebellar Basket Cell Terminal Pinceau in Adam11 Mutants Reveals Their Role in Ephaptic Control of Purkinje Cell Firing. The Journal of neuroscience : the official journal of the Society for Neuroscience, 11433-11444. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 20089912 | not listed | Ogawa, Y., et al. 2010. ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. The Journal of neuroscience : the official journal of the Society for Neuroscience, 1038-1048. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 29670100 | 1:250 | Yamagata, A., et al. 2018. Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22. Nature communications, 1546. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 22473675 | not listed | Dhanchak, A., et al. 2012. Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis. Annals of neurology, 601-613. |
| 20463223 | not listed | Thomas, R., et al. 2010. LGI1 is a Nogo receptor 1 ligand that antagonizes myelin-based growth inhibition. The Journal of neuroscience : the official journal of the Society for Neuroscience, 6607-6612. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 38194969 | not listed | Miyazaki, Y, et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634. |
| 34910912 | not listed | Yokoi, N., et al. 2021. 14-3-3 proteins stabilize LGI1-ADAM22 levels to regulate seizure thresholds in mice. Cell reports, 110107. |
| 30846310 | 1:200 | Tai, Y., et al. 2019. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron, 358-372. |
| 28452394 | not listed | Frank, R., et al. 2017. Hierarchical organization and genetically separable subfamilies of PSD95 postsynaptic supercomplexes. Journal of neurochemistry, 504-511. |
| 26269648 | 1:200 | Kole, M., et al. 2015. Selective Loss of Presynaptic Potassium Channel Clusters at the Cerebellar Basket Cell Terminal Pinceau in Adam11 Mutants Reveals Their Role in Ephaptic Control of Purkinje Cell Firing. The Journal of neuroscience : the official journal of the Society for Neuroscience, 11433-11444. |
| 25707359 | not listed | Chen, N., et al. 2015. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes. Biochimica et biophysica acta, 827-833. |
| 19701204 | 1:1000 | Zhou, Y., et al. 2009. Arrested maturation of excitatory synapses in autosomal dominant lateral temporal lobe epilepsy. Nature medicine, 1208-1214. |