Our Anti-AMIGO-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L86A/37. It is KO validated, detects human, mouse, and rat AMIGO-1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Amphoterin-induced protein 1, amphoterin-induced gene and open reading frame, Adhesion Molecule With Ig Like Domain 1 or Amigo-1 is encoded by the gene AMIGO1 and is a member of the AMIGO family. Amigo1 acts as a neuronal adhesion protein and contains leucine-rich repeat and immunoglobin domains. It is thought to play a role in cell to cell interactions and in neuronal plasticity/regenereation. Amigo1 is expressed in the nervous system and at low levels in liver, kidney, spleen, lung and heart.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L86A/37
IgG2b
ICC, IHC, WB
Mouse
Amigo1 Ali2 Amigo Kiaa1163
60-80 kDa depending on maturity/glycosylation
Fusion protein amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1 (accession number Q86WK6) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2315802
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Bishop, H.I., et al. 2018. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. Frontiers in Molecular Science, 1.
Mandikian, D., et al. 2014. Cell type-specific spatial and functional coupling between mammalian brain Kv2.1 K+ channels and ryanodine receptors.. Journal of Comparative Neurology, 3555-3574.
Mitchell, K.G., et al. 2023. High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Scientific Reports, 16200.
Maverick, E.E., et al. 2021. Kv2 channel-AMIGO β-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking. Journal of Cell Science, jcs256339.
Raja, R, et al. 2024. Insertion of a neomycin selection cassette in the Amigo1 locus alters gene expression in the olfactory epithelium leading to region-specific defects in olfactory receptor neuron development. Genesis, e23594.
