Anti-AMIGO-1 Antibody (L86A/37)

7 Citations

Our Anti-AMIGO-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L86A/37. It is KO validated, detects human, mouse, and rat AMIGO-1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.
Roll over image to zoom in Click on image to zoom
Adult rat hippocampus immunohistochemistry.
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with V5-tagged AMIGO-1, AMIGO-2, AMIGO-3 or untagged Kv2.1 plasmid and probed with L86A/37 TC supe (left) plasmid and probed with L86A/37 TC supe (left).
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and AMIGO-1 KO mouse brains probed with L86A/37 (left), K89/34 (middle) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Juha Kuja-Panula (University of Helsinki, Finland).

Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

Go to Full Product Details

Data Sheet
KO Validated KO Validated

SKU: 75-329

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

AMIGO-1
Amphoterin-induced protein 1, amphoterin-induced gene and open reading frame, Adhesion Molecule With Ig Like Domain 1 or Amigo-1 is encoded by the gene AMIGO1 and is a member of the AMIGO family. Amigo1 acts as a neuronal adhesion protein and contains leucine-rich repeat and immunoglobin domains. It is thought to play a role in cell to cell interactions and in neuronal plasticity/regenereation. Amigo1 is expressed in the nervous system and at low levels in liver, kidney, spleen, lung and heart.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L86A/37
IgG2b
ICC, IHC, WB
Mouse
Amigo1 Ali2 Amigo Kiaa1163
60-80 kDa depending on maturity/glycosylation
Fusion protein amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1 (accession number Q86WK6) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2315802
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Amphoterin-induced protein 1 (AMIGO-1) (Alivin-2)
Q80ZD8

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
327089171:500Dulla, C.G., et al. 2020. Regulation of neurogenesis in mouse brain by HMGB1.. Cells, 1714.
Immunohistochemistry: Mouse
PMID Dilution Publication
29403353not listedBishop, H.I., et al. 2018. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. Frontiers in Molecular Science, 1.
24962901not listedMandikian, D., et al. 2014. Cell type-specific spatial and functional coupling between mammalian brain Kv2.1 K+ channels and ryanodine receptors.. Journal of Comparative Neurology, 3555-3574.
Immunohistochemistry: Rat
PMID Dilution Publication
37758930not listedMitchell, K.G., et al. 2023. High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Scientific Reports, 16200.
Western Blot: Human
PMID Dilution Publication
341374431:1000 Maverick, E.E., et al. 2021. Kv2 channel-AMIGO β-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking. Journal of Cell Science, jcs256339.
Western Blot: Mouse
PMID Dilution Publication
385901461:5 (supe)Raja, R, et al. 2024. Insertion of a neomycin selection cassette in the Amigo1 locus alters gene expression in the olfactory epithelium leading to region-specific defects in olfactory receptor neuron development. Genesis, e23594.
327089171:1000Dulla, C.G., et al. 2020. Regulation of neurogenesis in mouse brain by HMGB1.. Cells, 1714.

Bulk Order Anti-AMIGO-1 Antibody

Related Products

Recently Viewed