Anti-Ankyrin-R Antibody (N388A/10)

Name: Anti-Ankyrin-R Antibody
Brand: NeuroMab
SKU: 75-380
Price: 319.0 USD
Availability: InStock

Our Anti-Ankyrin-R mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N388A/10. It is KO validated, detects human, mouse, and rat Ankyrin-R, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against crude membranes from adult rat brain (RBM) and wild-type (WT) and Ankyrin-R knockout (KO) mouse brains probed with N388A/10 (left) and K58/35 (right) TC supe. Mouse brains courtesy of Matthew Rasband (Baylor College of Medicine.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-380

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Ankyrin-R

Target Description

Ankyrin-1 or -Ankyrin-R is encoded by the gene ANK1. Ankyrin R is a scaffold protein that functions to link proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane cytoskeleton. In this manner, ankyrin family proteins play key roles in various processes including cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin R is found expressed in erythrocytes, skeletal muscle and brain. Diseases associated with this gene include Spherocytosis.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N388A/10

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

ANK1 ANK

Molecular Weight

200 kDa

Antigen

Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R (accession number P16157) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2491109

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Ankyrin-B or Ankyrin-G

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Ankyrin-1 (ANK-1) (Ankyrin-R) (Erythrocyte ankyrin)

UniProt Details

UniProt (Human): P16157
UniProt (Immunogen Species): P16157

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Immunoprecipitation: Mouse
PLA: Human
Western Blot: Human | Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
34785580	1:250	Stevens, S.R., et al. 2022. Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival. Journal of Neuroscience, 45337.
34180393	1:250	Stevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491.
32425157	1:500	Liu, C.H., et al. 2020. β spectrin-dependent and domain specific mechanisms for Na + channel clustering. Elife, e56629.
29963618	not listed	Taylor, A.M., et al. 2018. Simultaneous Ablation of Neuronal Neurofascin and Ankyrin G in Young and Adult Mice Reveals Age-Dependent Increase in Nodal Stability in Myelinated Axons and Differential Effects on the Lifespan. eNeuro, .
29907663	not listed	Saifetiarova, J., et al. 2018. Reorganization of Destabilized Nodes of Ranvier in β IV Spectrin Mutants Uncovers Critical Timelines for Nodal Restoration and Prevention of Motor Paresis. Journal of Neuroscience, 6267-6282.
28148727	not listed	Saifetiarova, J., et al. 2017. Early and Late Loss of the Cytoskeletal Scaffolding Protein, Ankyrin G Reveals Its Role in Maturation and Maintenance of Nodes of Ranvier in Myelinated Axons. Journal of Neuroscience, 2524-2538.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
34180393	5ul	Stevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491.

PLA: Human
PMID	Dilution	Publication
28387307	1:2	Genetet, S., et al. 2017. Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1). Scientific Reports, 46170.

Western Blot: Human
PMID	Dilution	Publication
30309849	not listed	Ladli, M., et al. 2019. Finely-tuned regulation of AMP-activated protein kinase is crucial for human adult erythropoiesis. Haematologica, 907-918.

Western Blot: Mouse
PMID	Dilution	Publication
34180393	1:500	Stevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491.
29963618	not listed	Taylor, A.M., et al. 2018. Simultaneous Ablation of Neuronal Neurofascin and Ankyrin G in Young and Adult Mice Reveals Age-Dependent Increase in Nodal Stability in Myelinated Axons and Differential Effects on the Lifespan. eNeuro, .

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Anti-Ankyrin-R Antibody (N388A/10)

SKU: 75-380

Product Details

Product Specific References for Applications and Species

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