Anti-AIFM1 (Ser-116), Phosphospecific Antibody

11 Citations

Our AIFM1 (Ser-116) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human AIFM1 (Ser-116) and is antigen affinity purified. It is great for use in WB.

Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).
Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).
Roll over image to zoom in Click on image to zoom
Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).
Western blotting shows KEAP1-PGAM5-AIFM1 pathway protein level regulation by ALT. SKOV-3 cells were treated with different concentrations (0, 16, 32 and 64 μM) of ALT for 24 h. Specifically AIFM1, P-AIFM1(Ser-116) (cat. AP5501), KEAP1, Nrf2, and PGAM5 levels were detected. B-Actin used as a control. Image from publication CC-BY-4.0. PMID: 37712005
Western blot analysis of protein expression of ASIC1, KEAP1, PGAM5, and AIFM1 phosphorylated at Ser116 (cat. AP5501, 1:1000) in NPCs after treatment for 30 min with 20 nM Psalmotoxin 1 (PcTx1) followed by 24 h of treatment with H2O (control) or 100 μM TBHP. Image from publication CC-BY-4.0. PMID: 38540291

Human
WB
Rabbit

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Data Sheet

SKU: AP5501

Volume: 100 µL
Price:
$340.00
Ships: 1-2 business days

Product Details

AIFM1 (Ser-116)
Apoptosis-inducing factor (AIFM1, AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis. AIFM1 is expressed as a 66 kDa precursor protein before being N-terminally cleaved to 62 kDa and localized to the mitochondrial intermembrane space. In response to apoptotic stimuli, AIFM1 is released from the mitochondrial intermembrane as a 57 kDa fragment that can translocate to the nucleus. Treatment of isolated nuclei with recombinant AIFM1 leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation. Studies of AIFM1 knockout mice have shown that the apoptotic activity of AIFM1 is cell type and stimuli-dependent. AIFM1 has been implicated in oxeiptosis, a non-inflammatory, caspase independent cell death pathway caused by oxidative stress. During oxeiptosis, increased reactive oxygen species cause the release of the phosphatase PGAM5 from KEAP1 leading to dephosphorylation of AIFM1 (Ser-116) and subsequent cell death. Thus, AIFM1 phosphorylation status at Ser-116 may be an important marker for cell death involving oxeiptosis.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
AIFM1
57/62/66
Phospho-AIFM1 (Ser-116) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-116 in human AIFM1. This site is conserved in rat and mouse AIFM1, but the amino acids surrounding the site are not well conserved. The site is not found in AIFM2 and AIFM3.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
The antibody was cross adsorbed to unphosphorylated AIFM1 (Ser-116) peptide before affinity purification using phospho-AIFM1 (Ser-116) peptide. This antibody antibody detects a triplet at 66, 62, and 57 kDa* protein on SDS-PAGE immunoblots of human Jurkat cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylated AIFM1.
Phosphorylated
Ser-116
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
PDCD8, AIF1, Apoptosis inducing factor 1, mitochondrial; Program cell death protein 8; striatal AIF
O95831

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
29255269not listedHolze, C, et al. 2018. Oxeiptosis, a ROS-induced caspase-independent apoptosis-like cell-death pathway. Nature immunology, 130-140.
Immunohistochemistry: Human
PMID Dilution Publication
377411301:200You, M, et al. 2023. 4-Octyl itaconate inhibits inflammation to attenuate psoriasis as an agonist of oxeiptosis. International Immunopharmacology, 110915.
Western Blot: Human
PMID Dilution Publication
39978447not listedZhu, J, et al. 2025. Mechanism of action of Danlou tablets affecting MAFLD via KEAP1-mediated oxeiptosis. Journal of Ethnopharmacology, 119521.
37712005not listedNasirzadeh, M, et al. 2023. Alantolactone triggers oxeiptosis in human ovarian cancer cells via Nrf2 signaling pathway. Biochemistry and Biophysics Reports, 101537.
37741130not listedYou, M, et al. 2023. 4-Octyl itaconate inhibits inflammation to attenuate psoriasis as an agonist of oxeiptosis. International immunopharmacology, 110915.
37712005not listedNasirzadeh, M, et al. 2023. Alantolactone triggers oxeiptosis in human ovarian cancer cells via Nrf2 signaling pathway. Biochemistry and Biophysics Reports, 101537.
36626243not listedTsui, KH, et al. 2023. Mitoquinone shifts energy metabolism to reduce ROS-induced oxeiptosis in female granulosa cells and mouse oocytes. Aging, 246-260.
351775861:1000Kang, P, et al. 2022. Oxeiptosis: a novel pathway of melanocytes death in response to oxidative stress in vitiligo. Cell death discovery, 70.
348720051:1000Wang, CX, et al. 2022. Auriculasin enhances ROS generation to regulate colorectal cancer cell apoptosis, ferroptosis, oxeiptosis, invasion and colony formation. Biochemical and Biophysical Research Communications, 99-106.
Western Blot: Mouse
PMID Dilution Publication
385402911:1000Chen, X, et al. 2024. Circular RNA CircFOXO3 Functions as a Competitive Endogenous RNA for Acid-Sensing Ion Channel Subunit 1 Mediating Oxeiptosis in Nucleus Pulposus. Biomedicines, 0.
36914635not listedPallichankandy, S, et al. 2023. Targeting oxeiptosis-mediated tumor suppression: a novel approach to treat colorectal cancers by sanguinarine. Cell death discovery, 94.

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