Our Anti-AMIGO-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L86/33. It is KO validated, detects human, mouse, and rat AMIGO-1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunoblot versus RBM and lysates of WT and AMIGO-1 KO mouse brains probed with L86/33 (left), K89/34 (middle) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Juha Kuja-Panula (University of Helsinki, Finland).
Amphoterin-induced protein 1, amphoterin-induced gene and open reading frame, Adhesion Molecule With Ig Like Domain 1 or Amigo-1 is encoded by the gene AMIGO1 and is a member of the AMIGO family. Amigo1 acts as a neuronal adhesion protein and contains leucine-rich repeat and immunoglobin domains. It is thought to play a role in cell to cell interactions and in neuronal plasticity/regenereation. Amigo1 is expressed in the nervous system and at low levels in liver, kidney, spleen, lung and heart.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L86/33
IgG2a
ICC, IHC, WB
Mouse
Amigo1 Ali2 Amigo Kiaa1163
60-80 kDa depending on maturity/glycosylation
Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1 (accession number Q86WK6) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2315765
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Hawkins, N.A., et al. 2021. Epilepsy and neurobehavioral abnormalities in mice with a dominant-negative KCNB1 pathogenic variant. Neurobiology Disease, 105141.
Bishop, H.I., et al. 2018. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons.. Frontiers in Molecular Neuroscience, 1.
