Anti-Ankyrin-G (Blotting) Antibody (N106/20)

27 Citations

Our Anti-Ankyrin-G (Blotting) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N106/20. It detects human, mouse, and rat Ankyrin-G (Blotting), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.
Roll over image to zoom in Click on image to zoom
Adult rat brain membrane immunoblot.
Lysates of rat cortical neurons treated with the indicated compounds were subjected to ABE to purify palmitoylated proteins. Levels of palmitoyl-AnkG-190 (right blot) and total AnkG (cat. 75-187) expression in parent lysates (left blot bottom blot) were detected with specific antibodies. Exclusion of NH2OH was used as a control for assay specificity. Image from publication CC-BY-4.0. PMID:36969554
Representative field of view (FOV) for near-simultaneous recording of calcium activity (Fluo-4) and axonal mitochondrial transport (mitoTracker Red) of primary mouse hippocampal neurons in sparse cultures. Arrows mark axonal fiber. Inset: MAP2 (green), AnkG (magenta) and Tau immunolabeling to verify axonal identity and origin. Arrow indicates axon initial segment. Dotted line indicates axonal path. image from publication, CC-BY-4.0. PMID: 39698051.

Human, Mouse, Non-Human Primate, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-187

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

Ankyrin-G (Blotting)
Ankyrin-G (ankyrin 3) is a member of the Ankyrin family which consists of Ankyrin R (1), Ankyrin B (2) and Ankyrin G (3) all of which are expressed in the nervous system. Ankyrins are structural proteins that aid in the attachment of integral membrane proteins with the spectrin-actin cytoskeleton, and thus targets voltage-gated sodium channels, potassium channels, cell adhesion molecules and others at the nodes of Ranvier and axonal initial segments (AIS). Variants in Ankyrin G have been linked to bipolar disorder.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N106/20
IgG1
ICC, IHC, WB
Mouse
ANK3
>200 kDa
Fusion protein ~1000 amino acids of Ankyrin-G (accession number E9PE32) produced recombinantly in E. Coli
Human, Mouse, Non-Human Primate, Rat
AB_2057718
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.115
WB: 1:500
IHC: 1:500
ICC: 1:500
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Ankyrin-3 (ANK-3) (Ankyrin-G)
E9PE32

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
396980511:250Venneman, T, et al. 2024. Neuronal activity inhibits mitochondrial transport only in synaptically connected segments of the axon. Frontiers in Cellular Neuroscience, 1509283.
260244781:50Yamankurt, G., et al. 2015. Exon organization and novel alternative splicing of Ank3 in mouse heart.. PLoS One, e0128177.
22632975not listedGaliano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-1139.
Immunocytochemistry: Rat
PMID Dilution Publication
31910261not listedLee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
31474508not listedFréal, A., et al. 2019. Feedback-driven assembly of the axon initial segment. Neuron, 305-321.
307233961:1000Alpizar, S.A., et al. 2019. Loss of neurofascin-186 disrupts alignment of AnkyrinG relative to its binding partners in the axon initial segment. Frontiers in Cellular Neuroscience, 1.
Immunohistochemistry: Mouse
PMID Dilution Publication
329177081:200Werginz, P., et al. 2020. Tailoring of the axon initial segment shapes the conversion of synaptic inputs into spiking output in OFF-α T retinal ganglion cells. Science Advances, eabb6642.
327363741:200Werginz, P., et al. 2020. The relationship between morphological properties and thresholds to extracellular electric stimulation in α RGCs. Journal of Neural Engineering, 045015.
327266311:200Holderith, N., et al. 2020. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Reports, 107968.
31910261not listedLee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
296762601:500Galliano, E., et al. 2018. Embryonic and postnatal neurogenesis produce functionally distinct subclasses of dopaminergic neuron. Elife, e32373.
22632975not listedGaliano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-1139.
Immunohistochemistry: Non-Human Primate
PMID Dilution Publication
241079391:3000Puthussery, T., et al. 2013. NaV1. 1 channels in axon initial segments of bipolar cells augment input to magnocellular visual pathways in the primate retina. . Journal of Neuroscience, 16045-16059.
Immunohistochemistry: Rat
PMID Dilution Publication
319348591:500Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na+ and KATP channels to the rat cardiac intercalated disc. Elife, e52373.
261958031:500Wefelmeyer, W., et al. 2015. Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output.. PNAS: USA, 9757-9762.
STORM: Rat
PMID Dilution Publication
319348591:50Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na+ and KATP channels to the rat cardiac intercalated disc. Elife, e52373.
Western Blot: Human
PMID Dilution Publication
27356871not listedSohn, P.D., et al. 2016. Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment.. Molecular Neurodegeneration, 47.
24462599not listedTrinh-Trang-Tan, M.M., et al. 2014. AlphaII-spectrin participates in the surface expression of cell adhesion molecule L1 and neurite outgrowth.. Exp Cell Research, 365-380.
Western Blot: Mouse
PMID Dilution Publication
31910261not listedLee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
308463101:200Tai, Y., et al. 2019. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron, 358-372.
299565861:500Dash, B., et al. 2018. Nonmuscle myosin II isoforms interact with sodium channel alpha subunits. . Molecular pain, 1744806918788638.
22632975not listedGaliano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-1139.
Western Blot: Rat
PMID Dilution Publication
36969554not listedPiguel, N.H., et al. 2023. Palmitoylation Controls the Stability of 190 kDa Ankyrin-G in Dendritic Spines and is Regulated by ZDHHC8 and Lithium. Frontiers in Molecular Neuroscience, 1144066.
319348591:2000Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na+ and KATP channels to the rat cardiac intercalated disc. Elife, e52373.
23025758not listedBaalman, K.L., et al. 2013. Blast wave exposure impairs memory and decreases axon initial segment length.. Journal of Neurotrauma, 741-751.
218936421:200Roussos, P., et al. 2011. Molecular and genetic evidence for abnormalities in the nodes of Ranvier in schizophrenia.. Archives Of General Psychiatry, 7-15.
21617128not listedSato, P.Y., et al. 2011. Interactions between ankyrin-G, Plakophilin-2, and Connexin43 at the cardiac intercalated disc.. Circulation Research, 193-201.

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