Anti-Ankyrin-R Antibody (N388A/10)

11 Citations

Our Anti-Ankyrin-R mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N388A/10. It is KO validated, detects human, mouse, and rat Ankyrin-R, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.
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Adult rat hippocampus immunohistochemistry.
Immunoblot against crude membranes from adult rat brain (RBM) and wild-type (WT) and Ankyrin-R knockout (KO) mouse brains probed with N388A/10 (left) and K58/35 (right) TC supe. Mouse brains courtesy of Matthew Rasband (Baylor College of Medicine.

Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet
KO Validated KO Validated

SKU: 75-380

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

Ankyrin-R
Ankyrin-1 or -Ankyrin-R is encoded by the gene ANK1. Ankyrin R is a scaffold protein that functions to link proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane cytoskeleton. In this manner, ankyrin family proteins play key roles in various processes including cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin R is found expressed in erythrocytes, skeletal muscle and brain. Diseases associated with this gene include Spherocytosis.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N388A/10
IgG2b
ICC, IHC, WB
Mouse
ANK1 ANK
200 kDa
Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R (accession number P16157) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2491109
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Does not cross-react with Ankyrin-B or Ankyrin-G
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Ankyrin-1 (ANK-1) (Ankyrin-R) (Erythrocyte ankyrin)
P16157

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
347855801:250Stevens, S.R., et al. 2022. Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival. Journal of Neuroscience, 45337.
341803931:250Stevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491.
324251571:500Liu, C.H., et al. 2020. β spectrin-dependent and domain specific mechanisms for Na + channel clustering. Elife, e56629.
29963618not listedTaylor, A.M., et al. 2018. Simultaneous Ablation of Neuronal Neurofascin and Ankyrin G in Young and Adult Mice Reveals Age-Dependent Increase in Nodal Stability in Myelinated Axons and Differential Effects on the Lifespan. eNeuro, .
29907663not listedSaifetiarova, J., et al. 2018. Reorganization of Destabilized Nodes of Ranvier in β IV Spectrin Mutants Uncovers Critical Timelines for Nodal Restoration and Prevention of Motor Paresis. Journal of Neuroscience, 6267-6282.
28148727not listedSaifetiarova, J., et al. 2017. Early and Late Loss of the Cytoskeletal Scaffolding Protein, Ankyrin G Reveals Its Role in Maturation and Maintenance of Nodes of Ranvier in Myelinated Axons. Journal of Neuroscience, 2524-2538.
Immunoprecipitation: Mouse
PMID Dilution Publication
341803935ulStevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491.
PLA: Human
PMID Dilution Publication
283873071:2Genetet, S., et al. 2017. Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1). Scientific Reports, 46170.
Western Blot: Human
PMID Dilution Publication
30309849not listedLadli, M., et al. 2019. Finely-tuned regulation of AMP-activated protein kinase is crucial for human adult erythropoiesis. Haematologica, 907-918.
Western Blot: Mouse
PMID Dilution Publication
341803931:500Stevens, S.R., et al. 2021. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3. 1b K+ channels. . Elife, e66491.
29963618not listedTaylor, A.M., et al. 2018. Simultaneous Ablation of Neuronal Neurofascin and Ankyrin G in Young and Adult Mice Reveals Age-Dependent Increase in Nodal Stability in Myelinated Axons and Differential Effects on the Lifespan. eNeuro, .

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