Anti-ANO5/TMEM16E Antibody (N421A/85)

Our Anti-ANO5/TMEM16E mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N421A/85. It detects human ANO5/TMEM16E, and is purified by Protein A chromatography. It is great for use in ICC, WB.



SKU: 75-410

Volume: 100 µL
1-2 business days
Price:
Sale price$385.00

Product Details

ANO5/TMEM16E
Anoctamin-5, Transmembrane Protein 16E or -ANO5/TMEM16E is encoded by the gene ANO5. ANO5 belongs to the anoctamin family of membrane proteins many of which are calcium-activated chloride channels. ANO5 does not exhibit this channel activity. ANO5 is expressed in brain, heart, kidney, lung, skeletal muscle as well as other tissues. Diseases associated with ANO5 include Gnathodiaphyseal Dysplasia and Miyoshi Muscular Dystrophy 3.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N421A/85
IgG1
ICC, IHC, WB
Mouse
ANO5 GDD1 TMEM16E
110 kDa
Fusion protein amino acids 1-299 (cytoplasmic N-terminus) of human ANO5 (accession number Q75V66) produced recombinantly in E. Coli
Human, Mouse
AB_2531893
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
ICC: 1:100
Unconjugated
Does not cross-react with ANO6/TMEM16F
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Anoctamin-5 (Gnathodiaphyseal dysplasia 1 protein) (Transmembrane protein 16E)

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
296653211:100Xu, J., et al. 2018. A novel ANO5 splicing variant in a LGMD2L patient leads to production of a truncated aggregation-prone Ano5 peptide. Journal of Pathology, Clinical Research, 135-142.
Immunohistochemistry: Human
PMID Dilution Publication
28489263not listedVihola, A., et al. 2017. Diagnostic anoctamin-5 protein defect in patients with ANO5-mutated muscular dystrophy.. Neuropathology and Applied Neurobiology, 441-448.
Western Blot: Human
PMID Dilution Publication
36951953not listedSchmaier, A.A., et al. 2023. TMEM16E regulates endothelial cell procoagulant activity and thrombosis. Journal of Clinical Investigation, e163808.
36292621not listedSoontrapa, P., et al. 2022. Anoctamin 5 (ANO5) Muscle Disorders: A Narrative Review. Genes (Basel), 1736.
349634851:1000Li, H., et al. 2021. BVES is a novel interactor of ANO5 and regulates myoblast differentiation. Cell & bioscience, 11(1), pp.1-10.. Cell & Bioscience, 1-10.
29665321not listedXu, J., et al. 2018. A novel ANO5 splicing variant in a LGMD2L patient leads to production of a truncated aggregation-prone Ano5 peptide. Journal of Pathology, Clinical Research, 135-142.
28489263not listedVihola, A., et al. 2017. Diagnostic anoctamin-5 protein defect in patients with ANO5-mutated muscular dystrophy.. Neuropathology and Applied Neurobiology, 441-448.
Western Blot: Mouse
PMID Dilution Publication
269116751:100Griffin, D.A., et al. 2016. Defective membrane fusion and repair in Anoctamin5-deficient muscular dystrophy.. Human Molecular Genetics, 1900-1911.
Additional Publications: Unspecified
PMID Publication
33818761Mavillard, F., et al. 2021. Novel ANO5 intronic Roma variant alters splicing causing muscular dystrophy. Clinical Genetics, 106-110.

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