Anti-Arginase I (ARG1) Antibody
Our Anti-Arginase I (ARG1) primary antibody from PhosphoSolutions is chicken polyclonal. It detects guinea pig, human, mouse, and rat Arginase I (ARG1) and is total IgY fraction. It is great for use in WB, IHC.
SKU: 146-ARG1
Product Details
Arginase I (ARG1)
Arginase I, a cytosolic enzyme, is highly expressed in the liver and is a component of the urea cycle (Jenkinson et al, 1996). There are two isoforms of arginase, both catalyze the conversion of arginine to ornithine and urea, but differ based on subcellular localization, tissue distribution, and enzymatic properties (Jenkinson et al, 1996). Arginase I has been identified in macrophages in response to various pathogen exposures causing an upregulation of inducible nitric oxide synthase, iNOS, yielding increased levels of nitric oxide (Morris et al, 1998) and inflammation (Poljakovic et al, 2007).
Total IgY fraction
Polyclonal
IgY
IHC, WB
Chicken
ARG1
35/38 kDa
Full-length recombinant rat liver arginase I expressed in and purified from E. Coli.
Guinea Pig, Human, Mouse, Rat
AB_2732850
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Total IgY fraction.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
IHC: 1:500
IHC: 1:500
Unconjugated
Specific for endogenous levels of the ~35-38 kDa arginase I protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
A I antibody, Al antibody, ARG 1 antibody, arg1 antibody, ARGI1_HUMAN antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase liver antibody, Arginase-1 antibody, Arginase1 antibody, Liver type arginase antibody, Liver-type arginase antibody, Type I arginase antibody
UniProt (Human): P05089
UniProt (Immunogen Species): P07824
UniProt (Immunogen Species): P07824
29221
Product Specific References for Applications and Species
Immunohistochemistry: Rat | ||
PMID | Dilution | Publication |
17365572 | 1:500 | Poljakovic, M., et al. 2007. Cell-and isoform-specific increases in arginase expression in acute silica-induced pulmonary inflammation. Journal of Toxicology and Environmental Health, Part A, 70(2), pp.118-127. |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
9814991 | not listed | Morris, S., et al. 1998. Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells. American Journal of Physiology-Endocrinology And Metabolism, 275(5), pp.E740-E747. |
Product Specific Protocols
- Western Blot Protocol: Download