Anti-Arginase I (ARG1) Antibody

Name: Anti-Arginase I (ARG1) Antibody
Brand: PhosphoSolutions
SKU: 146-ARG1
Price: 445.0 USD
Availability: InStock

3 Citations

Our Anti-Arginase I (ARG1) primary antibody from PhosphoSolutions is chicken polyclonal. It detects guinea pig, human, mouse, and rat Arginase I (ARG1) and is total IgY fraction. It is great for use in WB, IHC.

Western blot of mouse liver lysate showing specific immunolabeling of the ~35, 38 kDa arginase I protein.

Immunofluorescent image of mouse retina specifically labeling arginase (cat. 146-ARG, 1:500, green) and CD206 (red). The blue nuclear stain is DAPI. The image was kindly provided by Sarah Gough, University of Miami Miller School of Medicine.

Species Reactivity

Guinea Pig, Human, Mouse, Rat

Applications

IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: 146-ARG1

Volume: 100 µL

Variant

Price:

$445.00

Quantity:

Product Details

Target

Arginase I (ARG1)

Target Description

Arginase I, a cytosolic enzyme, is highly expressed in the liver and is a component of the urea cycle (Jenkinson et al, 1996). There are two isoforms of arginase, both catalyze the conversion of arginine to ornithine and urea, but differ based on subcellular localization, tissue distribution, and enzymatic properties (Jenkinson et al, 1996). Arginase I has been identified in macrophages in response to various pathogen exposures causing an upregulation of inducible nitric oxide synthase, iNOS, yielding increased levels of nitric oxide (Morris et al, 1998) and inflammation (Poljakovic et al, 2007).

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

IHC, WB

Host Species

Chicken

Gene Name

ARG1

Molecular Weight

35/38 kDa

Antigen

Full-length recombinant rat liver arginase I expressed in and purified from E. Coli.

Species Reactivity

Guinea Pig, Human, Mouse, Rat

Antibody Registry ID

AB_2732850

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Total IgY fraction.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~35-38 kDa arginase I protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

A I antibody, Al antibody, ARG 1 antibody, arg1 antibody, ARGI1_HUMAN antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase liver antibody, Arginase-1 antibody, Arginase1 antibody, Liver type arginase antibody, Liver-type arginase antibody, Type I arginase antibody

UniProt Details

UniProt (Human): P05089
UniProt (Immunogen Species): P07824

NCBI Gene ID

29221

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication

39293691	1:100	Soliman, E., et al. 2024. Skull bone marrow-derived immune cells infiltrate the injured cerebral cortex and exhibit anti-inflammatory properties. Brain, Behavior, and Immunity, 244-253.

Immunohistochemistry: Rat
PMID	Dilution	Publication
17365572	1:500	Poljakovic, M., et al. 2007. Cell-and isoform-specific increases in arginase expression in acute silica-induced pulmonary inflammation. Journal of Toxicology and Environmental Health, Part A, 70(2), pp.118-127.

Western Blot: Mouse
PMID	Dilution	Publication
9814991	not listed	Morris, S., et al. 1998. Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells. American Journal of Physiology-Endocrinology And Metabolism, 275(5), pp.E740-E747.

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Product Specific Protocols

Western Blot Protocol: Download

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Anti-Arginase I (ARG1) Antibody

SKU: 146-ARG1

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Arginase I (ARG1) Antibody

Product Specific Protocols

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