Anti-Asc (Tyr-144), Phosphospecific Antibody

12 Citations

Our Asc (Tyr-144) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Asc (Tyr-144) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.
Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.
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Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.
Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University)
Immunoprecipitation analysis of ASC Y144 (cat. AP5631) phosphorylation in mouse bone marrow-derived macrophages (BMDMs) infected with Mock, PRV-WT, PRV-UL4mut for 12 h. Image from publication CC-BY-4.0. PMID: 39110798
Western blot image of BMDM cells treated with LPS +MSU in the presence or absence of SLC3037. The cell extracts was subjected to immunoblot analysis using anti-ASC and anti-phospho ASC-tyr 144 (Cat no AP5631, 1:1000). Image from publication, CC-BY-4.0. PMID: 38371945.

Human, Mouse, Rat
ICC, WB
Rabbit

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Data Sheet

SKU: AP5631

Volume: 100 µL
Price:
$340.00
Ships: 1-2 business days

Product Details

Asc (Tyr-144)
Host- and pathogen-associated cytoplasmic double-stranded DNA triggers the activation of a NALP3-independent inflammasome, which activates caspase-1, leading to maturation of pro-interleukin-1beta and inflammation. Several studies have isolated AIM2 (absent in melanoma 2) as a candidate cytoplasmic-DNA-sensing protein that contains an N-terminal pyrin domain and C-terminal oligonucleotide binding domain. A screen for transcripts induced by interferon-beta identified AIM2 gene expression. AIM2 protein bound double-stranded DNA, recruited the inflammasome adaptor ASC, and localized to ASC containing speckles. AIM2 and ASC form a pyroptosome, which induces pyroptotic cell death mediated by caspase-1. Asc can be phosphorylated at Tyr-144 in a Syk and JNK-dependent manner. This phosphorylation is critical for Asc speck formation and Caspase-1 activation.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
Pycard
22
Asc (Tyr-144) phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-144 in mouse Asc. This peptide sequence is highly conserved in human and rat Asc.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
Unconjugated
This antibody was cross-adsorbed to unphosphorylated Asc (Tyr-144) peptide before affinity purification using phospho-Asc (Tyr-144) peptide. The antibody detects phosphorylated Asc (Tyr-144) in the inflammasome. In J774 macrophage cells primed with LPS and treated with nigericin, the antibody colocalized with an inflammasome marker, caspase-1 inhibitor (FAM-YVAD-FMK). In addition, the antibody detects a 20 kDa Asc protein in western blots of J774A.1 mouse macrophages treated with pervanadate.
Phosphorylated
Tyr-144
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Caspase, PYCARD, AIM, ASC, CARD5, TMS1
Q9EPB4

Product Specific References for Applications and Species

ELISA: Mouse
PMID Dilution Publication
32963032not listedLin, YH, et al. 2020. Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury. Molecular & cellular proteomics: MCP, 2030-2047.
Immunohistochemistry: Mouse
PMID Dilution Publication
32963032not listedLin, YH, et al. 2020. Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury. Molecular & cellular proteomics: MCP, 2030-2047.
Western Blot: Human
PMID Dilution Publication
33568399not listedGavrilin, MA, et al. 2021. cAbl Kinase Regulates Inflammasome Activation and Pyroptosis via ASC Phosphorylation. Journal of immunology (Baltimore, Md. : 1950), 1329-1336.
30157949not listedFuruya, MY, et al. 2018. Tofacitinib inhibits granulocyte-macrophage colony-stimulating factor-induced NLRP3 inflammasome activation in human neutrophils. Arthritis research & therapy, 196.
Western Blot: Mouse
PMID Dilution Publication
39316625not listedZhang, X., et al. 2024. Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation. PLoS Pathogens, e1012546.
389532851:1000Kang, H, et al. 2024. ER-to-lysosome Ca2+ refilling followed by K+ efflux-coupled store-operated Ca2+ entry in inflammasome activation and metabolic inflammation. eLife, .
383719451:1000Park, K, et al. 2023. A novel NLRP3 inhibitor as a therapeutic agent against monosodium urate-induced gout. Frontiers in Immunology, 1307739.
354745991:1000Jung, ES, et al. 2022. Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway. Aging cell, e13623.
32963032not listedLin, YH, et al. 2020. Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury. Molecular & cellular proteomics: MCP, 2030-2047.
310179811:1000Dubois, H, et al. 2019. Nlrp3 inflammasome activation and Gasdermin D-driven pyroptosis are immunopathogenic upon gastrointestinal norovirus infection. PLoS pathogens, e1007709.
29686531not listedKwak, SB, et al. 2018. Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation. Mediators of inflammation, 6054069.
24721477not listedHoyt, LR, et al. 2016. Ethanol and Other Short-Chain Alcohols Inhibit NLRP3 Inflammasome Activation through Protein Tyrosine Phosphatase Stimulation. Journal of immunology (Baltimore, Md. : 1950), 1322-1334.

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