Our β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)] rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)] and is antigen affinity purified. It is great for use in WB, ICC.
Western blot analysis of Hct116 src transformed cells (20 µg/lane) serum starved overnight or treated with pervanadate (1 mM) for 30 min. The blot was probed with anti-β-Catenin or anti-β-Catenin (Tyr-142).
β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
CTNNB1
92
Phospho-β-Catenin (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human β-Catenin. This peptide sequence has one amino acid difference from a sequence around tyrosine 133 of human γ-Catenin. These human sequences are highly conserved in rat and mouse β- and γ-Catenins.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
ICC: 1:100
Unconjugated
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho-β-Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of Hct116 src-transformed cells treated with pervanadate, but not in control cells. Similar results were observed in pervanadate-treated A431 and human endothelial cells.
Phosphorylated
Tyr-142
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Beard Jr, R.S., et al. 2011. Hyperhomocysteinemia increases permeability of the blood-brain barrier by NMDA receptor-dependent regulation of adherens and tight junctions. Blood, 2007-2014.
Hamada-Kawaguchi, N., et al. 2015. Btk29A-mediated tyrosine phosphorylation of armadillo/β-catenin promotes ring canal growth in Drosophila oogenesis. PLoS One, e0121484.
Kline, A, et al. 2018. The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber. Developmental biology, 99-112.
Qi, F., et al. 2013. Continuous exposure to chrysotile asbestos can cause transformation of human mesothelial cells via HMGB1 and TNF-α signaling. American Journal of Pathology, 1654-1666.
Huynh, H, et al. 2019. Sorafenib/MEK inhibitor combination inhibits tumor growth and the Wnt/β‑catenin pathway in xenograft models of hepatocellular carcinoma. International journal of oncology, 1123-1133.
