Anti-β-Catenin (Tyr-142) [γ-Catenin (Tyr-133)], Phosphospecific Antibody

Name: Anti-β-Catenin (Tyr-142) [γ-Catenin (Tyr-133)], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP1081
Price: 340.0 USD
Availability: InStock

9 Citations

Our β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)] rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)] and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal β-Catenin and rabbit polyclonal β-Catenin (Tyr-142) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of Hct116 src transformed cells (20 µg/lane) serum starved overnight or treated with pervanadate (1 mM) for 30 min. The blot was probed with anti-β-Catenin or anti-β-Catenin (Tyr-142).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: CP1081

Volume: 100 µL

Variant

Price:

$340.00

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Product Details

Target

β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)]

Target Description

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

CTNNB1

Molecular Weight

Antigen

Phospho-β-Catenin (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human β-Catenin. This peptide sequence has one amino acid difference from a sequence around tyrosine 133 of human γ-Catenin. These human sequences are highly conserved in rat and mouse β- and γ-Catenins.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho-β-Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of Hct116 src-transformed cells treated with pervanadate, but not in control cells. Similar results were observed in pervanadate-treated A431 and human endothelial cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-142

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Catenin beta1, CTNNB1, catenin

UniProt ID

P35222

Product Specific References for Applications and Species

ELISA: Mouse
Immunocytochemistry: Drosophila | Mouse
Immunohistochemistry: Drosophila
Western Blot: Human | Mouse | Rat

ELISA: Mouse
PMID	Dilution	Publication
21705496	1:500	Beard Jr, R.S., et al. 2011. Hyperhomocysteinemia increases permeability of the blood-brain barrier by NMDA receptor-dependent regulation of adherens and tight junctions. Blood, 2007-2014.

Immunocytochemistry: Drosophila
PMID	Dilution	Publication
25803041	1:200	Hamada-Kawaguchi, N., et al. 2015. Btk29A-mediated tyrosine phosphorylation of armadillo/β-catenin promotes ring canal growth in Drosophila oogenesis. PLoS One, e0121484.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
25266662	not listed	Tsuneki, M., et al. 2014. Adhesion molecule-mediated hippo pathway modulates hemangioendothelioma cell behavior. Molecular and Cellular Biology, 4485-4499.

Immunohistochemistry: Drosophila
PMID	Dilution	Publication
29753016	1:200	Kline, A, et al. 2018. The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber. Developmental biology, 99-112.

Western Blot: Human
PMID	Dilution	Publication
34252536	not listed	Boudria, R, et al. 2021. Regulatory interplay between Vav1, Syk and β-catenin occurs in lung cancer cells. Cell signalling, 110079.
24160326	not listed	Qi, F., et al. 2013. Continuous exposure to chrysotile asbestos can cause transformation of human mesothelial cells via HMGB1 and TNF-α signaling. American Journal of Pathology, 1654-1666.

Western Blot: Mouse
PMID	Dilution	Publication
30747223	not listed	Huynh, H, et al. 2019. Sorafenib/MEK inhibitor combination inhibits tumor growth and the Wnt/β‑catenin pathway in xenograft models of hepatocellular carcinoma. International journal of oncology, 1123-1133.

Western Blot: Rat
PMID	Dilution	Publication
30988299	1:2000	Wong, HLX, et al. 2019. Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling. Nature communications, 1745.
22558232	not listed	Krejci, P., et al. 2012. Receptor tyrosine kinases activate canonical WNT/β-catenin signaling via MAP kinase/LRP6 pathway and direct β-catenin phosphorylation. PLoS One, e35826.

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Anti-β-Catenin (Tyr-142) [γ-Catenin (Tyr-133)], Phosphospecific Antibody

SKU: CP1081

Product Details

Product Specific References for Applications and Species

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