Anti-BAF53a Antibody (N336B/83)

Name: Anti-BAF53a Antibody
Brand: NeuroMab
SKU: 75-330
Price: 329.0 USD
Availability: InStock

Our Anti-BAF53a mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N336B/83. It detects human, mouse, and rat BAF53a, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-330

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

BAF53a

Target Description

Actin-like protein 6A, or BAF Complex 53 KDa Subunit (BAF53a) is encoded by the gene ACTL6A and is a member of actin-related proteins family. It is a subunit of the neuron specific chromatin remodeling complex (nBAF complex). BAF53a is thought to be involved in transcriptional activation and repression of chromatin remodeling. BAF53a is a subunit of the neural progenitors-specific chromatin remodeling complex (npBAF complex). BAF53a is expressed in many different cell types. Diseases associated with ACTL6A include Coffin-siris syndrome and Brachymorphism-Onychodysplasia-Dysphalangism Syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N336B/83

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

ACTL6A BAF53 BAF53A INO80K

Molecular Weight

53 kDa

Antigen

Fusion protein amino acids 43-119 (MVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKSEASLHP, actin subdomain 2) of human BAF53a (accession number O96019) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315809

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with BAF53b

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Actin-like protein 6A (53 kDa BRG1-associated factor A) (Actin-related protein Baf53a) (ArpNbeta) (BRG1-associated factor 53A) (BAF53A) (INO80 complex subunit K)

UniProt ID

O96019

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28270570	not listed	Yoo, M., et al. 2017. BAF53b, a Neuron-Specific Nucleosome Remodeling Factor, Is Induced after Learning and Facilitates Long-Term Memory Consolidation. Journal of Neuroscience, 3686-3697.

Western Blot: Mouse
PMID	Dilution	Publication
36289231	not listed	Fulton, S.L., et al. 2022. Rescue of deficits by Brwd1 copy number restoration in the Ts65Dn mouse model of Down syndrome. Nature Communications, 6384.

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Anti-BAF53a Antibody (N336B/83)

SKU: 75-330

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-BAF53a Antibody

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