Anti-Bassoon Antibody (L124/59)

9 Citations

Our Anti-Bassoon mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L124/59. It detects mouse and rat Bassoon, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L124/59 (magenta), rabbit mAb PSD-95 (Cell Signaling
Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L124/59 (magenta), rabbit mAb PSD-95 (Cell Signaling #3450, green) and Hoechst nuclear stain (blue). The insert shows three consecutive sections through the synapse that is marked with a white box.
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Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L124/59 (magenta), rabbit mAb PSD-95 (Cell Signaling
Adult rat hippocampus immunohistochemistry.

Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-491

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

Bassoon
Bassoon is a large (420-kDa) scaffolding protein specifically localized at the active zone of presynaptic nerve terminals. Bassoon is involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses (Richter K. et al., 1999).
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L124/59
IgG2b
ICC, IHC, WB
Mouse
Bsn
400 kDa for full-length protein, many smaller bands possible depending on sample used
Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli
Mouse, Rat
AB_2744564
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
IHC: 1:500
ICC: 1:500
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Protein bassoon
O88778

Product Specific References for Applications and Species

dSTORM: Mouse
PMID Dilution Publication
366081231:1000Lin, P.Y., et al. 2023. Neurexin-2: An inhibitory neurexin that restricts excitatory synapse formation in the hippocampus. Science Advances, eadd8856.
Immunocytochemistry: Mouse
PMID Dilution Publication
316656291:500Moretto, E., et al. 2019. TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. Cell Reports, 1130-1146.e8.
Immunocytochemistry: Rat
PMID Dilution Publication
316656291:500Moretto, E., et al. 2019. TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering. Cell Reports, 1130-1146.e8.
25918374not listedVarea, O., et al. 2015. Synaptic abnormalities and cytoplasmic glutamate receptor aggregates in contactin associated protein-like 2/Caspr2 knockout neurons.. PNAS USA, 6176-81.
Immunohistochemistry: Mouse
PMID Dilution Publication
398442861:250Tran, K, et al. 2025. APOE Christchurch enhances a disease-associated microglial response to plaque but suppresses response to tau pathology. Molecular Neurodegeneration, 9.
385066341:250Butler, CA, et al. 2024. The Abca7V1613M variant reduces Aβ generation, plaque load, and neuronal damage. Alzheimer's & Dementia, .
384601211:250Garcia-Agudo, LF, et al. 2024. BIN1K358R suppresses glial response to plaques in mouse model of Alzheimer's disease. Alzheimer's & Dementia, .
363342651:500Allen, B.D., et al. 2022. Elucidating The Neurological Mechanism Of The Flash Effect In Juvenile Mice Exposed To Hypofractionated Radiotherapy. Neuro-Oncology, .
PLA: Mouse
PMID Dilution Publication
384922282ug/mlVerstraelen, P, et al. 2024. Integrated staging of morphofunctional connectivity in neuronal cultures. STAR Protocols, 102957.

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