Anti-C-MYC Epitope Tag Antibody

8 Citations

This product is an unlabeled, affinity-purified chicken anti-C-MYC epitope antibody.

Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
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Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.

ELISA, ICC, IHC, IP, WB
Chicken

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Data Sheet

SKU: ET-MY100

Volume: 1 mL
Price:
$304.00
Ships: 1-2 business days

Product Details

C-MYC Epitope Tag Antibody
The synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL).
Affinity Purified IgY
100 µg/mL (based on absorbance at 280 nm)
Polyclonal
IgY
ELISA, ICC, IHC, IP, WB
Chicken
N/A
Synthetic peptide
AB_2313514
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.
Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) as an anti-microbial agent.
WB: 1:500-1:1000
IHC: 1:100-1:250
ICC: 1:100-1:250
Antibodies were analyzed using ELISA and Western Blots using an N-terminal C-MYC-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs

Product Specific References for Applications and Species

Flow Cytometry: Human
PMID Dilution Publication
12070135not listedCunningham, S.A., et al. 2002. JAM2 interacts with α4β1: facilitation by JAM3. Journal of Biological Chemistry, 27589-27592.
Flow Cytometry: Yeast
PMID Dilution Publication
390802823ug/mlFreschlin, C.R., et al. 2024. Neural network extrapolation to distant regions of the protein fitness landscape. Nature Communications, 6405.
35174856not listedHeinzelman, P., et al. 2022. Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species. Protein engineering, design & selection : PEDS, .
Immunocytochemistry: Human
PMID Dilution Publication
246108141:1000Slavoff, S.A., et al. 2014. A human short open reading frame (sORF)-encoded polypeptide that stimulates DNA end joining. Journal of Biological Chemistry, 10950-10957.
17686003not listedPonniah, G., et al. 2007. State of differentiation defines buccal epithelial cell affinity for cross‐linking to Candida albicans Hwp1. Journal of Oral Pathology and Medicine, 456-467.
Immunoprecipitation: Rat
PMID Dilution Publication
28957379not listedGrimaldo L, et al. 2017. Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels. Plos One, e0185289.
Immunoprecipitation: Yeast
PMID Dilution Publication
339793915ug/mlHeinzelman, P., et al. 2021. Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein. PLoS One, e0251585.
Western Blot: Rat
PMID Dilution Publication
289573791:500Grimaldo L, et al. 2017. Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels. Plos One, e0185289.

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