Anti-CALB1/calbindin Antibody (L109/57)

5 Citations

Our Anti-CALB1/calbindin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L109/57. It detects human, mouse, and rat CALB1/calbindin, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cerebellum with L109/57 (red) and DAPI (blue). Left panel is a single 70 nm section imaged with a 20X objective, with a scale bar of 50 µm. Right panel is a MAX projection of 10 serial 70 nm sections imaged with a 63X objective, with a scale bar of 20 µm. Images courtesy of Kristina Micheva (Stanford).
Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cerebellum with L109/57 (red) and DAPI (blue). Left panel is a single 70 nm section imaged with a 20X objective, with a scale bar of 50 µm. Right panel is a MAX projection of 10 serial 70 nm sections imaged with a 63X objective, with a scale bar of 20 µm. Images courtesy of Kristina Micheva (Stanford).
Roll over image to zoom in Click on image to zoom
Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cerebellum with L109/57 (red) and DAPI (blue). Left panel is a single 70 nm section imaged with a 20X objective, with a scale bar of 50 µm. Right panel is a MAX projection of 10 serial 70 nm sections imaged with a 63X objective, with a scale bar of 20 µm. Images courtesy of Kristina Micheva (Stanford).
Adult rat hippocampus immunohistochemistry.
Adult rat brain membrane immunoblot.
Representative maximum intensity projections from WT (black) and NPC1I1061T (red) mouse cerebellar sagittal sections co-immunolabeled for Calbindin(cat. 75-448, 2ug/ml) , CaV1.2 (cat. 75-257, 5ug/ml) and KV2.1 (cat. 75-014, 10ug/ml). Image from publication CC-BY-4.0. PMID:37507375

Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

Go to Full Product Details

Data Sheet

SKU: 75-448

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

CALB1/calbindin
Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993). Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014).
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L109/57
IgG1
ICC, IHC, WB
Mouse
CALB1 CAB27
30 kDa
Fusion protein amino acids 1-261 (full-length) of human CALB1 (accession number P05937) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2686913
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
IHC: 1:250
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Calbindin (Calbindin D28) (D-28K) (Vitamin D-dependent calcium-binding protein, avian-type)
P05937

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
391033181:200Han, X., et al. 2024. Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex. Nature Communications, 6648.
375073752ug/mlCasas, M., et al. 2023. NPC1-dependent alterations in KV2.1–CaV1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease. Nature Communications, 4553.
347855801:250Stevens, S.R., et al. 2022. Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival. Journal of Neuroscience, 2-15.
Immunohistochemistry: Rat
PMID Dilution Publication
374913161:500Nagaraja, R.Y., et al. 2023. Synapse-specific Defects in Synaptic Transmission in the Cerebellum of W246G Mutant ELOVL4 Rats- A Model of Human SCA34. The Journal of neuroscience : the official journal of the Society for Neuroscience, 5963-5974.
32726631not listedHolderith, N., et al. 2020. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Reports, 107968.

Bulk Order Anti-CALB1/calbindin Antibody

Related Products

Recently Viewed