Anti-Cav1.2 Ca2+ Channel Antibody (N263/31)

Our Anti-Cav1.2 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N263/31. It detects human, mouse, and rat Cav1.2 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in ICC, WB.



SKU: 75-257

Volume: 100 µL
1-2 business days
Price:
Sale price$329.00

Product Details

Cav1.2 Ca2+ channel
Voltage-dependent L-type calcium channel subunit alpha-1C or Cav1.2 calcium channel (other names include as CACNA1C, CACH2, CACN2, CACNL1A1, CCHL1A1 ) is a calcium channel encoded by the gene CACNA1C. It is a member of the L type voltage dependent calcium channel family. These calcium channels mediate the influx of calcium ions into a cell upon membrane polarization. Cav1.2 is expressed in many tissues including smooth muscle, liver, kidney brain and heart. In brain, it can be detected in the hippocampus and brain cortex in the post-synaptic density and in neuronal cell bodies. Calcium channels are involved in many cell processes including muscle contraction, neurotransmitter release, gene expression, cell division and cell death. Mutations in the Cav1.2 gene have been associated with Timothy syndrome, Brugada syndrome 3 and Long QT syndrome 8.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N263/31
IgG2b
ICC, IHC, WB
Mouse
Cacna1c Cach2 Cacn2 Cacnl1a1 Cchl1a1
240 kDa
Fusion protein amino acids 808-874 (cytoplasmic loop between repeat II and III) of rat Cav1.2 (accession number Q5S007) produced recombinantly in E. Coli
Guinea Pig, Human, Mouse, Rabbit, Rat
AB_11000167
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:1000
ICC: 1:100
Unconjugated
Does not cross-react with Cav1.3
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Voltage-dependent L-type calcium channel subunit alpha-1C (Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle) (Rat brain class C) (RBC) (Voltage-gated calcium channel subunit alpha Cav1.2)

Product Specific References for Applications and Species

Fluorescence Resonance Energy Transfer: Rat
PMID Dilution Publication
270766161:100Bannister, J.P., et al. 2016. Rab25 influences functional Cav1.2 channel surface expression in arterial smooth muscle cells. American Journal of Physiology. Cell Physiology, C885-C895.
Immunocytochemistry: Guinea Pig
PMID Dilution Publication
314034021:200Morgenstern, T.J., et al. 2019. A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVβ subunits. Elife, e49253.
Immunocytochemistry: Mouse
PMID Dilution Publication
377027871:100Akin, EJ, et al. 2023. ANO1, CaV1.2, and IP3R form a localized unit of EC-coupling in mouse pulmonary arterial smooth muscle. The Journal of General Physiology, 0.
374384791:100Cserne Szappanos, H., et al. 2023. Cytoskeletal Disarray Increases Arrhythmogenic Vulnerability During Sympathetic Stimulation in a Model of Hypertrophic Cardiomyopathy. Scientific Reports, 11296.
347502631:5 (supe)Vierra, N.C., et al. 2021. Regulation of neuronal excitation-transcription coupling by Kv2.1-induced clustering of somatic L-type Ca2+ channels at ER-PM junctions. PNAS: USA, .
3308233910ug/mlPrada, M.P., et al. 2020. AKAP5 complex facilitates purinergic modulation of vascular L-type Ca2+ channel CaV1.2. Nature Communications, 5303.
316638501:5 (supe)Vierra, N.C., et al. 2019. Kv2. 1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in mammalian neurons. Elife, e49953.
Immunocytochemistry: Rat
PMID Dilution Publication
344319811:100Isensee, J., et al. 2021. Depolarization induces nociceptor sensitization by CaV1.2-mediated PKA-II activation. Journal of Cell Biology, .
27364017not listedEichel, C.A., et al. 2016. Lateral Membrane-Specific MAGUK CASK Down-Regulates NaV1.5 Channel in Cardiac Myocytes. Circulation Research, 544-56.
Immunohistochemistry: Human
PMID Dilution Publication
281194641:1000Nystoriak, M.A., et al. 2017. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Scientific Signaling, eaaf9647.
Immunohistochemistry: Mouse
PMID Dilution Publication
375073755ug/mlCasas, M., et al. 2023. NPC1-dependent alterations in KV2.1–CaV1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease. Nature Communications, 4553.
347502631:5 (supe)Vierra, N.C., et al. 2021. Regulation of neuronal excitation-transcription coupling by Kv2.1-induced clustering of somatic L-type Ca2+ channels at ER-PM junctions. PNAS: USA, .
Immunoprecipitation: Rat
PMID Dilution Publication
246813471:500Liu, W., et al. 2014. KCNE2 modulates cardiac L-type Ca(2+) channel.. Journal of Molecular Cellular Cardiology, 208-218.
PLA: Human
PMID Dilution Publication
281194641:1000Nystoriak, M.A., et al. 2017. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Scientific Signaling, eaaf9647.
PLA: Mouse
PMID Dilution Publication
38860414not listedXu, B, et al. 2024. Differential Downregulation of β1-Adrenergic Receptor Signaling in the Heart. Journal of the American Heart Association, e033733.
STED: Rabbit
PMID Dilution Publication
29549309not listedZhang, X.D., et al. 2018. Coupling of SK channels, L-type Ca2+ channels, and ryanodine receptors in cardiomyocytes. Scientific Reports, 4670.
Western Blot: Guinea Pig
PMID Dilution Publication
267644821:500Nassal, D.M., et al. 2016. Myocardial KChIP2 expression in guinea pig resolves an expanded electrophysiologic role. PLoS One, e0146561.
Western Blot: Human
PMID Dilution Publication
244016931:200Duan, L., et al. 2014. Stem cell derived basal forebrain cholinergic neurons from Alzheimer''s disease patients are more susceptible to cell death.. Molecular Neurdegeneration, 3.
238580111:250Narayanan, D., et al. 2013. Smooth muscle cell transient receptor potential polycystin-2 (TRPP2) channels contribute to the myogenic response in cerebral arteries.. Journal of Physiology, 5031-5046.
Western Blot: Mouse
PMID Dilution Publication
32442021not listedGupte, R., et al. 2020. Glucose-6-phosphate dehydrogenase increases Ca2+ currents by interacting with Cav1.2 and reducing intrinsic inactivation of the L-type calcium channel. American Journal of Physiology. Heart and Circulatory Physiology, H144-H158.
Western Blot: Rat
PMID Dilution Publication
334532401:1000Nasu, F., et al. 2021. Azelnidipine treatment reduces the expression of Cav1.2 protein. Life Sciences, 119043.
31337710not listedJiang, M., et al. 2019. S-Palmitoylation of junctophilin-2 is critical for its role in tethering the sarcoplasmic reticulum to the plasma membrane. Journal of Biological Chemistry, 13487-13501.
256103721:1000Perissinotti, P.P., et al. 2015. Calcium current homeostasis and synaptic deficits in hippocampal neurons from Kelch-like 1 knockout mice.. Frontiers in Cellular Neuroscience, 444.
247039041:1000Perissinotti, P.P., et al. 2014. Down-regulation of endogenous KLHL1 decreases voltage-gated calcium current density.. Cell Calcium, 269-280.
238580111:250Narayanan, D., et al. 2013. Smooth muscle cell transient receptor potential polycystin-2 (TRPP2) channels contribute to the myogenic response in cerebral arteries.. Journal of Physiology, 5031-5046.
23568894not listedBannister, J.P., et al. 2013. The voltage-dependent L-type Ca2+ (CaV1.2) channel C-terminus fragment is a bi-modal vasodilator. Journal of Physiology, 2987-2998.

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