Our Anti-Cav1.3 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L48A/9. It detects human, mouse, and rat Cav1.3 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Voltage-dependent L-type calcium channel subunit alpha-1D, or Cav1.3 calcium channel is a calcium channel encoded by the gene CACNA1D. It is a member of the L type voltage dependent calcium channel family. These calcium channels mediate the influx of calcium ions into a cell upon membrane polarization. Cav1.3 is expressed primarily in the membrane of the heart, brain (cerebral cortex, hippocampus, basal ganglia and thalamus). and pancreatic islets. Calcium channels are involved in many cell processes including muscle contraction, neurotransmitter release, gene expression, cell division and cell death. Diseases associated with Cav1.3 include Primary Aldosteronism, Seizures, Sinoatrial Node Dysfunction and Deafness.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L48A/9
IgG2a
ICC, IHC, IP, WB
Mouse
Cacna1d Cach3 Cacn4 Cacnl1a2 Cchl1a2
250 kDa
Synthetic peptide amino acids 859-875 (N-terminus) of rat Cav1.3 (accession number P27732)
Human, Mouse, Rat
AB_2069388
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.41
Unconjugated
No cross-reactivity against Cav1.2
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Hettiarachchi, N.T., et al. 2009. alpha-Synuclein modulation of Ca2+ signaling in human neuroblastoma (SH-SY5Y) cells.. Journal of Neurochemistry, 1192-1201.
Lu, L., et al. 2015. Regulation of gene transcription by voltage-gated L-type calcium channel, Cav1.3.. The Journal of Biological Chemistry, 4663-4676.
Yamasaki, S., et al. 2012. A novel role of the L-type calcium channel α1D subunit as a gatekeeper for intracellular zinc signaling: zinc wave.. PLoS One, e39654.
Perissinotti, P.P., et al. 2015. Calcium current homeostasis and synaptic deficits in hippocampal neurons from Kelch-like 1 knockout mice.. Frontiers in Neuroscience, 444.
