Anti-Cav1.3 Ca2+ Channel Antibody (N38/8)

Our Anti-Cav1.3 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N38/8. It detects human, mouse, and rat Cav1.3 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.



SKU: 75-080

Volume: 100 µL
Price:
Sale price$329.00
Ships: 1-2 business days

Product Details

Cav1.3 Ca2+ channel
Voltage-dependent L-type calcium channel subunit alpha-1D, or Cav1.3 calcium channel is a calcium channel encoded by the gene CACNA1D. It is a member of the L type voltage dependent calcium channel family. These calcium channels mediate the influx of calcium ions into a cell upon membrane polarization. Cav1.3 is expressed primarily in the membrane of the heart, brain (cerebral cortex, hippocampus, basal ganglia and thalamus). and pancreatic islets. Calcium channels are involved in many cell processes including muscle contraction, neurotransmitter release, gene expression, cell division and cell death. Diseases associated with Cav1.3 include Primary Aldosteronism, Seizures, Sinoatrial Node Dysfunction and Deafness.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N38/8
IgG1
ICC, IHC, IP, WB
Mouse
Cacna1d Cach3 Cacn4 Cacnl1a2 Cchl1a2
>220 kDa
Fusion protein amino acids 2025-2161 (C-terminus) of rat Cav1.3 (accession number P27732) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_10673964
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.10
WB: 1:1000
IHC: 1:100
ICC: 1:100
Unconjugated
Does not cross-react with Cav1.2
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Voltage-dependent L-type calcium channel subunit alpha-1D (Calcium channel, L type, alpha-1 polypeptide, isoform 2) (Rat brain class D) (RBD) (Voltage-gated calcium channel subunit alpha Cav1.3)

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
23576958not listedGinger, M., et al. 2013. Three-dimensional tracking and analysis of ion channel signals across dendritic arbors. Frontiers in neural circuits, 61.
232295761:100Liedtke, W., et al. 2013. Highly conductive carbon nanotube matrix accelerates developmental chloride extrusion in central nervous system neurons by increased expression of chloride transporter KCC2. Small (Weinheim an der Bergstrasse, Germany), 1066-1075.
212817071:100Chen, W., et al. 2011. Complex distribution patterns of voltage-gated calcium channel α-subunits in the spiral ganglion. Hearing research, 52-68.
Immunocytochemistry: Rat
PMID Dilution Publication
232295761:100Liedtke, W., et al. 2013. Highly conductive carbon nanotube matrix accelerates developmental chloride extrusion in central nervous system neurons by increased expression of chloride transporter KCC2. Small (Weinheim an der Bergstrasse, Germany), 1066-1075.
Immunohistochemistry: Human
PMID Dilution Publication
270988371:500Xie, C., et al. 2016. Regulation of aldosterone secretion by Cav1.3. Scientific reports, 24697.
237713391:100Hurley, M., et al. 2013. Parkinson's disease is associated with altered expression of CaV1 channels and calcium-binding proteins. Brain : a journal of neurology, 2077-2097.
19778450not listedBernstein, S., et al. 2009. Expressed sequence tag analysis of adult human optic nerve for NEIBank: identification of cell type and tissue markers. BMC neuroscience, 121.
Immunohistochemistry: Mouse
PMID Dilution Publication
279325041:100Radhakrishnan, V., et al. 2017. Expression of Cav1.3 calcium channel in the human and mouse colon: posttranscriptional inhibition by IFNγ. American journal of physiology. Gastrointestinal and liver physiology, G77-G84.
Immunohistochemistry: Rat
PMID Dilution Publication
24033980not listedNúñez-Santana, F., et al. 2014. Surface L-type Ca2+ channel expression levels are increased in aged hippocampus. Aging cell, 111-120.
Proximity Ligation Assay: Mouse
PMID Dilution Publication
3889107610ug/mlBelghazi, M, et al. 2024. High-Resolution Proteomics Unravel a Native Functional Complex of Cav1.3, SK3, and Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Midbrain Dopaminergic Neurons. Cells, .
Western Blot: Human
PMID Dilution Publication
239130041:500Azizan, E., et al. 2013. Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension. Nature genetics, 1055-1060.
23771339not listedHurley, M., et al. 2013. Parkinson's disease is associated with altered expression of CaV1 channels and calcium-binding proteins. Brain : a journal of neurology, 2077-2097.
19778450not listedBernstein, S., et al. 2009. Expressed sequence tag analysis of adult human optic nerve for NEIBank: identification of cell type and tissue markers. BMC neuroscience, 121.
Western Blot: Mouse
PMID Dilution Publication
27932504not listedRadhakrishnan, V., et al. 2017. Expression of Cav1.3 calcium channel in the human and mouse colon: posttranscriptional inhibition by IFNγ. American journal of physiology. Gastrointestinal and liver physiology, G77-G84.
25538241not listedLu, L., et al. 2015. Regulation of gene transcription by voltage-gated L-type calcium channel, Cav1.3. The Journal of biological chemistry, 4663-4676.
228921111:1000Zou, J., et al. 2012. The expression of whirlin and Cav1.3α₁ is mutually independent in photoreceptors. Vision research, 53-59.
22133878not listedGoonasekera, S., et al. 2012. Decreased cardiac L-type Ca²⁺ channel activity induces hypertrophy and heart failure in mice. The Journal of clinical investigation, 280-290.
219404471:1000Schierberl, K., et al. 2011. Cav1.2 L-type Ca²⁺ channels mediate cocaine-induced GluA1 trafficking in the nucleus accumbens, a long-term adaptation dependent on ventral tegmental area Ca(v)1.3 channels. The Journal of neuroscience : the official journal of the Society for Neuroscience, 13562-13575.
21281707not listedChen, W., et al. 2011. Complex distribution patterns of voltage-gated calcium channel α-subunits in the spiral ganglion. Hearing research, 52-68.
Western Blot: Rat
PMID Dilution Publication
24033980not listedNúñez-Santana, F., et al. 2014. Surface L-type Ca2+ channel expression levels are increased in aged hippocampus. Aging cell, 111-120.
234575531:4 (supe)Bissig, B., et al. 2013. Diminished vision in healthy aging is associated with increased retinal L-type voltage gated calcium channel ion influx. PloS one, e56340.

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