Anti-CD133 (Prominin1) Antibody
Our CD133 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD133 and is antigen affinity purified. It is great for use in WB, IHC.
![Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. Image from the following publ](http://www.antibodiesinc.com/cdn/shop/files/36898-icc-1_e4793dea-d62c-45ed-881c-4fd5e1eac349_{width}x.jpg?v=1722538456)
![Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. Image from the following publ](http://www.antibodiesinc.com/cdn/shop/files/36898-ihc-1_37b49510-3ed7-40ce-b44c-bb8d06d5f9d0_{width}x.jpg?v=1722538456)
![Immunohistochemistry of human hepatocarcinoma (HC) tissue showing specific staining of CD133.](http://www.antibodiesinc.com/cdn/shop/files/36898-ihc-2_4a338df9-6846-44aa-8b9b-ea082e5c1c1c_{width}x.jpg?v=1722538456)
![Chromatin Immunoprecipitation showing staining of CD133 in Bone Marrow Mononuclear Cells by immunofluorescence.](http://www.antibodiesinc.com/cdn/shop/files/36898-ip-1_ffd996ce-d940-4909-bcef-5f8241058baa_{width}x.jpg?v=1722538456)
![Western Blot of Y79 cells showing specific immunolabeling of human CD133. Band is at ~120 kDa due to protein glycosylation.](http://www.antibodiesinc.com/cdn/shop/files/36898-wb-1_d0b3ca35-1036-4202-965d-4cca87ba8085_{width}x.jpg?v=1722538456)
Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. Image from the following publ
SKU: 36898
Product Details
CD133
Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.
Antigen Affinity Purified
Polyclonal
IgG
IHC, WB
Rabbit
PROM1
Synthetic peptide corresponding to amino acids from an internal sequence of the human Prominin-1 (CD133) protein.
Human
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
PBS + 0.02% NaN3
WB: 1:500-1:1000
WB Brain: 1:500-1:1000
IHC: 1:100-1:250
WB Brain: 1:500-1:1000
IHC: 1:100-1:250
Reacts with an internal sequence of the human Prominin-1 protein. Sequence is 87% identical in mouse and rat.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
PROM1, Antigen AC133, Prominin-like protein 1, PROML1, MSTP061
UniProt (Human): O43490
UniProt (Immunogen Species): O43490
UniProt (Immunogen Species): O43490