Our Anti-CD75 (Transferrin receptor) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects CD75 and is Protein L purified. It is great for use in WB, IHC.
Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa CD75 protein.
CD75, also known as β-galactoside α2,6-sialyltransferase, is a specific carbohydrate antigen that is predominantly expressed on B cell lymphocytes. β-galactoside α2,6-sialyltransferase is an enzyme that catalyzes the addition of sialic acid to growing carbohydrate chains of glycoproteins (Guy, K., et al, 1991). CD75 is a major component of the lymphoid cell surface that serves as a ligand for cell adhesion molecules (Paulson, J.C., et al, 1989). The LN-1 clone reacts positively with red blood cell precursors of the bone marrow, ciliated epithelial cells of the bronchus, distal tubular cells of the kidney, and ductal cells from several organs including the breast and prostate (Epstein, A.L., et al, 1984). CD75 has been shown to be superior to other B cell markers in detecting lymphocyte predominant cells through their cytoplasmic and membraneous staining, especially in typical nodular lymphocyte-predominant Hodgkin lymphoma patterns (Carbone, A., et al, 2014).
Protein L purified
Monoclonal
LN1
IgM
IHC, WB
Mouse
ST6GAL1
35 kDa
Nuclei isolated from pokeweed mitogen-stimulated human peripheral blood lymphocytes.
Human
AB_2890167
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Protein L purified culture supernatant.
PBS
WB: 1:100
IHC: 1:100
Unconjugated
Specific for endogenous levels of the ~35 kDa CD75 protein.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
