Our Anti-cdc2 (Tyr15) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and Xenopus cdc2 (Tyr15) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of human T47D cells showing specific immunolabeling of the ~34 kDa cdc2 phosphorylated at Tyr15 (Control, Lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase, λ-Ptase (1200 units for 30 min).
Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr-15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
CDK1
34 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 of rat cdc2, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse, Xenopus
Rat, Zebrafish
AB_2492052
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~34 kDa cdc2 protein phosphorylated at Tyr15. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Tyr15
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Cdc 2 antibody, Cdc2 antibody, CDC28A antibody, CDK-1 antibody, CDK 1 antibody, CDK1 antibody, CDK1_HUMAN antibody, CDKN1 antibody, CELL CYCLE CONTROLLER CDC2 antibody, Cell division control protein 2 antibody, Cell division control protein 2 homolog antibody, Cell division cycle 2 G1 to S and G2 to M antibody, Cell division protein kinase 1 antibody, Cell Divsion Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, Cyclin-dependent kinase 1 antibody, DKFZp686L20222 antibody, MGC111195 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, P34CDC2 antibody
Dranovsky, A., Vincent, I., Gregori, L., Schwarzman, A., Colflesh, D., Enghild, J., Strittmatter, W., Davies, P. and Goldgaber, D., 2001. Cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer’s disease pathology. Neurobiology of aging, 22(4), pp.517-528. PMID: 11445251
Konishi, Y. and Bonni, A., 2003. The E2F–Cdc2 cell-cycle pathway specifically mediates activity deprivation-induced apoptosis of postmitotic neurons. Journal of Neuroscience, 23(5), pp.1649-1658. PMID: 12629169
Nakamizo, A., Inamura, T., Inoha, S., Amano, T., Ochi, H., Ikezaki, K. and Fukui, M., 2002. Suppression of Cdc2 Dephosphorylation at the Tyrosine 15 Residue during Nitrosourea-induced G 2 M phase Arrest in Glioblastoma Cell Lines. Journal of neuro-oncology, 59(1), pp.7-13. PMID: 12222840
Okano, H.J., Pfaff, D.W. and Gibbs, R.B., 1996. Expression of EGFR-, p75NGFR-, and PSTAIR (cdc2)-like immunoreactivity by proliferating cells in the adult rat hippocampal formation and forebrain. Developmental neuroscience, 18(3), pp.199-209. PMID: 8894448
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