Anti-ChAT (Choline Acetyltransferase) Antibody
Our Anti-ChAT (Choline Acetyltransferase) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects chicken, guinea pig, human, mouse, non-human primate, and rat ChAT and is neat serum. It is great for use in WB, IHC.
Chicken, Guinea Pig, Human, Mouse, Non-Human Primate, Rat
IHC, WB
Rabbit
![Trial Size](https://cdn.shopify.com/s/files/1/0512/5793/4009/files/icon-trial-size_430x.png)
SKU: 316-ChAT
Product Details
ChAT
Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer's disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
Neat serum
Polyclonal
IgG
IHC, WB
Rabbit
CHAT
70 kDa
Native choline acetyltransferase purified from human placenta.
Chicken, Guinea Pig, Human, Mouse, Non-Human Primate, Rat
AB_2492056
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Neat serum.
Neat serum
WB: 1:1000
IHC: 1:100
IHC: 1:100
Unconjugated
Specific for endogenous levels of the ~70 kDa choline acetyltransferase protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Acetyl CoA choline O acetyltransferase antibody, Acetyl CoA:choline O acetyltransferase antibody, ChAT antibody, CHOACTase antibody, Choline acetylase antibody, choline acetyltransferase antibody, Choline O acetyltransferase antibody, Choline O-acetyltransferase antibody, CLAT_HUMAN antibody, CMS1A antibody, CMS1A2 antibody, EC 2.3.1.6 antibody, OTTHUMP00000019583 antibody, OTTHUMP00000019584 antibody
UniProt (Human): P28329
UniProt (Immunogen Species): P28329.
UniProt (Immunogen Species): P28329.
1103
Product Specific Protocols
- Western Blot Protocol: Download
General References
- Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459. PMID: 8434662
- Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31.PMID: 10613493
- Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123. PMID: 10488813