Anti-Che-1 (AATF) (Ser477) Antibody

2 Citations

Our Anti-Che-1 (AATF) (Ser477) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Che-1 (AATF) (Ser477) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Roll over image to zoom in Click on image to zoom
Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Human
WB
Rabbit

Go to Full Product Details

Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: p142-477

Volume: 100 µL
1-2 business days
Price:
$445.00

Product Details

Che-1 (AATF) (Ser477)
Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
AATF
66 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1, conjugated to keyhole limpet hemocyanin (KLH).
Human
Bovine, Canine, Mouse, Non-Human Primate, Rat, Sheep, Xenopus
AB_2492057
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~66 kDa Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser477
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
AATF antibody, AATF_HUMAN antibody, Apoptosis antagonizing transcription factor antibody, Apoptosis-antagonizing transcription factor antibody, BFR2 antibody, CHE 1 antibody, CHE1 antibody, DED antibody, Protein AATF antibody, Rb binding protein Che 1 antibody, Rb-binding protein Che-1 antibody
Q9NY61
26574

Product Specific References for Applications and Species

Western Blot: Human
PMID Dilution Publication
27012205 not listed Liu, M., et al. 2016. Che-1 gene silencing induces osteosarcoma cell apoptosis by inhibiting mutant p53 expression. Biochemical and biophysical research communications, 473(1), pp.168-173.
25996291 not listed Desantis, A., et al. 2015. Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53. Cell death & disease, 6(5), p.e1764.

Bulk Order Anti-Che-1 (AATF) (Ser477) Antibody

Product Specific Protocols

Related Products

Recently Viewed