Our Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, rabbit, rat, and sheep CNP (2,3-cyclic nucleotide-3-phosphodiesterase) and is neat serum. It is great for use in WB, IHC.
Immunochemical staining of saline treated mouse cortex cryosections showing specific labeling of CNP(cat. 325-CNP, green, 1:500). The blue is staining DNA. The bisected longitudinal mouse brain was fixed for 18h in 4% paraformaldehyde, washed, and cryoprotected in 30% sucrose overnight. The cryosections were antigen retrieved in citrate buffer, blocked in 20% normal goat serum for 1 hr. Photo courtesy of Robert Wine.
2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Neat serum
Neat serum
WB: 1:1000
WB Brain: 1:1000
IHC: 1:500-1:1000
Unconjugated
Specific for endogenous levels of the ~46 kDa CNP protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Ramaswamy, S.G., et al. 1990. A novel autoantibody from a rabbit preimmune serum that immunostains myelinated nerves of the brain. Brain research bulletin, 25(1), pp.193-197.
González-Fernández, E., et al. 2018. PTEN negatively regulates the cell lineage progression from NG2+ glial progenitor to oligodendrocyte via mTOR-independent signaling. eLife, 7, p.e32021.
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